[1]李雨晴),刘红春),朱 峰).转染miRNA-145、黏结合蛋白多糖-2 siRNA对膀胱癌T24细胞增殖、分化的影响[J].郑州大学学报(医学版),2019,(02):262-267.[doi:10.13705/j.issn.1671-6825.2018.06.051]
 LI Yuqing),LIU Hongchun),ZHU Feng).Effects of miRNA-145 transfection and syndecan-2 siRNA transfection on cell proliferation and differentiation of bladder cancer T24 cells[J].JOURNAL OF ZHENGZHOU UNIVERSITY(MEDICAL SCIENCES),2019,(02):262-267.[doi:10.13705/j.issn.1671-6825.2018.06.051]
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转染miRNA-145、黏结合蛋白多糖-2 siRNA对膀胱癌T24细胞增殖、分化的影响()
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《郑州大学学报(医学版)》[ISSN:1671-6825/CN:41-1340/R]

卷:
期数:
2019年02期
页码:
262-267
栏目:
应用研究
出版日期:
2019-03-20

文章信息/Info

Title:
Effects of miRNA-145 transfection and syndecan-2 siRNA transfection on cell proliferation and differentiation of bladder cancer T24 cells
作者:
李雨晴1)刘红春23)朱 峰2)
1)河南中医药大学第二临床学院检验科 郑州 450046 2)郑州大学第一附属医院检验科 郑州 450052 3)郑州大学检验系 郑州 450052
Author(s):
LI Yuqing1)LIU Hongchun23)ZHU Feng2)
1)Department of Clinical Laboratory,the Second Clinical Medical College,Henan University of Chinese Medicine,Zhengzhou 450046 2)Department of Clinical Laboratory,the First Affiliated Hospital,Zhengzhou University,Zhengzhou 450052 3)Department of Inspection,Zhengzhou University,Zhengzhou 450052
关键词:
膀胱癌 微小RNA-145 黏结合蛋白多糖-2 增殖 分化
Keywords:
bladder cancer miRNA-145 syndecan-2 proliferation differentiation
分类号:
R737.14
DOI:
10.13705/j.issn.1671-6825.2018.06.051
摘要:
目的:探讨促进膀胱癌T24细胞中微小RNA-145(miRNA-145)的表达、抑制黏结合蛋白多糖-2(syndecan-2)的表达对细胞增殖、分化的影响。方法:实验Ⅰ中T24细胞转染miRNA-145,以未处理细胞为空白对照。采用MTT法检测转染24、48和72 h后细胞增殖情况,荧光定量PCR检测转染72 h后syndecan-2,鳞状细胞标志物(P63、TP73和CK5),腺状细胞标志物(MUC-1、MUC-3),神经内分泌细胞标志物(NSE、UCHL-1、CGA)及干细胞标志物(NANOG、OCT3、SOX2和 E2F4)mRNA的表达。实验Ⅱ中T24细胞转染Syndecan-2 siRNA,以未处理细胞为空白对照。转染 72 h后,采用衰老相关β-半乳糖苷酶法检测细胞衰老情况,TUNEL法检测细胞凋亡情况,荧光定量PCR 检测细胞分化标志物和NANOG mRNA的表达。结果:与空白对照组相比,转染miRNA-145组细胞增殖和syndecan-2表达被抑制,细胞分化标志物和干细胞标志物mRNA表达均增加(P<0.05)。沉默Syndecan-2表达后,细胞衰老形态学变化明显,细胞衰老率升高,细胞分化标志物和NANOG mRNA表达增加(P<0.05)。结论:促进miRNA-145表达可抑制Syndecan-2表达,从而加速细胞衰老和分化,抑制癌细胞增殖。
Abstract:
Aim:To investigate the effects of upregulating the expression of micro RNA-145(miRNA-145)and downregulating the expression of syndecan-2 on cell proliferation and differentiation of bladder cancer T24 cells.Methods: In experiment Ⅰ,bladder cancer T24 cells were transfected with miRNA-145 and the cells not treated were blank control.MTT assay was used to detect cell proliferation at 24,48,72 hour after transfection, and the mRNA expressions of syndecan-2, stem cell markers and cell differentiation markers were detected by Real-time PCR. In experiment Ⅱ,T24 cells were transfected with syndecan-2 siRNA and the cells not treated were blank control.After 72 hour transfection,cell senescence was detected by SA-β-gal assay, cell apoptosis was detected by TUNEL assay,and the mRNA expressions of cell differentiation markers and NANOG were detected by Real-time PCR.Results:Compared with blank control, cell proliferation and the expression of syndecan-2 mRNA in cells transfected with miRNA-145 were inhibited,while the mRNA expressions of cell differentiation markers and stem cell differentiation markers were increased(P<0.05).Senile cells were observed in the cells transfected with syndecan-2 siRNA and the mRNA expressions of cell differentiation markers and NANOG were increased compared with blank control(P<0.05).Conclusion: Upregulation of miRNA-145 inhibits cell proliferation and induces senescence and differentiation by inhibiting the expression of syndecan-2.

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备注/Memo

备注/Memo:
【基金项目】河南省科技攻关基金资助项目(172102310028); 河南省教育厅重点科技攻关项目(18A320007); 河南省卫计委医学科技攻关项目(201602042)
【作者简介】刘红春,通信作者,女,1963年1月生,博士,主任医师,教授,研究方向:肿瘤基因诊断,E-mail:xingyunerliu@163.com
更新日期/Last Update: 2019-03-20