[1]张 斌,刘 岳,林建清,等.RNAi抑制NOB1表达对肝癌BEL-7402细胞增殖及p38MAPK磷酸化水平的影响[J].郑州大学学报(医学版),2019,(02):271-275.[doi:10.13705/j.issn.1671-6825.2018.09.175]
 ZHANG Bin,LIU Yue,LIN Jianqing,et al.Effects of inhibiting NOB1 expression by RNAi on proliferation and phosphorylation of p38MAPK in hepatocellular carcinoma BEL-7402 cells[J].JOURNAL OF ZHENGZHOU UNIVERSITY(MEDICAL SCIENCES),2019,(02):271-275.[doi:10.13705/j.issn.1671-6825.2018.09.175]
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RNAi抑制NOB1表达对肝癌BEL-7402细胞增殖及p38MAPK磷酸化水平的影响()
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《郑州大学学报(医学版)》[ISSN:1671-6825/CN:41-1340/R]

卷:
期数:
2019年02期
页码:
271-275
栏目:
应用研究
出版日期:
2019-03-20

文章信息/Info

Title:
Effects of inhibiting NOB1 expression by RNAi on proliferation and phosphorylation of p38MAPK in hepatocellular carcinoma BEL-7402 cells
作者:
张 斌刘 岳林建清倪亚安毛盛名
广州医科大学附属第六医院(清远市人民医院)肝胆外科 广东清远 511518
Author(s):
ZHANG BinLIU YueLIN JianqingNI Ya'anMAO Shengming
Department of Hepatobiliary Surgery, the Sixth Affiliated Hospital, Guangzhou Medical University(Qingyuan People's Hospital), Qingyuan,Guangdong 511518
关键词:
肝癌 BEL-7402细胞 NOB1 p38MAPK
Keywords:
liver carcinoma BEL-7402 cell NOB1 p38MAPK
分类号:
R735.7
DOI:
10.13705/j.issn.1671-6825.2018.09.175
摘要:
目的:探讨RNAi下调Nin1结合蛋白(NOB1)表达对肝癌细胞增殖及p38丝裂原活化蛋白激酶(p38MAPK)磷酸化水平的影响。方法:以肝癌BEL-7402细胞作为研究对象,将肝癌细胞分为空白对照组、siRNA-NC组、NOB1 siRNA组3组,其中siRNA-NC组、NOB1 siRNA组分别为稳定感染阴性对照慢病毒和NOB1 siRNA慢病毒的肝癌细胞,空白对照组为不做慢病毒感染的肝癌细胞。用qRT-PCR和Western blot检测干扰效果。CCK-8法测定细胞增殖情况,流式细胞仪检测细胞凋亡情况,Western blot法检测细胞中磷酸化p38MAPK和活化的Caspase-3蛋白的表达情况。结果:NOB1 siRNA组细胞中NOB1表达水平、细胞增殖活性降低,细胞凋亡率升高,磷酸化p38MAPK和活化的Caspase-3蛋白表达水平升高,与空白对照组、siRNA-NC组比较,差异均有统计学意义(P<0.05)。结论:下调NOB1表达具有抑制BEL-7402细胞增殖、诱导细胞凋亡、促进细胞中p38MAPK磷酸化的作用。
Abstract:
Aim:To investigate the effects of down-regulation of Nin one binding protein(NOB1)expression by RNAi on the proliferation and phosphorylation of p38MAPK in hepatocellular carcinoma cells.Methods:Hepatocellular carcinoma BEL-7402 cells were divided into three groups:blank control group, siRNA-NC group and NOB1 siRNA group. In siRNANC group and NOB1 siRNA group,BEL-7402 cells were infected with negative control lentivirus and NOB1 siRNA lentivirus respectively. The blank control group was cells without lentivirus infection. The interference effects were detected by qRT-PCR and Western blot. Cell proliferation activity was measured by CCK-8 assay.Flow cytometry was used to detect apoptosis. Western blot was used to detect the level of phosphorylated p38MAPK and activated Caspase-3 proteins in cells.Results:Compared with blank control group and siRNA-NC group,in NOB1 siRNA group, the expression of NOB1 and cell proliferation activity were decreased,apoptotic rate was increased,and the phosphorylated p38MAPK and activated Caspase-3 protein levels were increased(P<0.05).Conclusion:Down-regulation of NOB1 expression could inhibit the proliferation of BEL-7402 cells, induce apoptosis, and promote the phosphorylation of p38MAPK.

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备注/Memo

备注/Memo:
【基金项目】广东省自然科学基金资助项目(2016A030313365)
【作者简介】张斌,通信作者,男,1974年4月生,博士,副主任医师,研究方向:肝胆外科,E-mail:1554406618@qq.com
更新日期/Last Update: 2019-03-20