JI Penghui),DUAN Shuyin),LI Zunshui),et al.Value of support vector machine model based on tumor markers in aided diagnosis of lung cancer[J].JOURNAL OF ZHENGZHOU UNIVERSITY(MEDICAL SCIENCES),2019,(03):398-402.[doi:10.13705/j.issn.1671-6825.2018.11.047]





Value of support vector machine model based on tumor markers in aided diagnosis of lung cancer
纪鹏慧1)段书音1)李尊税1)王 静2)吴拥军1)
1)郑州大学公共卫生学院卫生毒理学教研室 郑州 450001 2)郑州大学第一附属医院呼吸内科 郑州 450052
JI Penghui1)DUAN Shuyin1)LI Zunshui1)WANG Jing2)WU Yongjun1)
1)Department of Health Toxicology,College of Public Health,Zhengzhou University,Zhengzhou 450001 2)Department of Respiratory Medicine,the First Affiliated Hospital,Zhengzhou University,Zhengzhou 450052
肿瘤标志物 肺癌 支持向量机 辅助诊断
tumor marker lung cancer support vector machine aided diagnosis
目的:探讨支持向量机模型结合肿瘤标志物联合检测在肺癌辅助诊断中的价值。方法:选择肺癌患者180例、肺良性疾病患者243例,采用放射免疫法检测血清中癌胚抗原(CEA)、神经元特异性烯醇化酶(NSE)和胃泌素的含量; 采用改良的间苯二酚显色法测定血清中唾液酸含量; 采用电感耦合等离子体质谱法测定血清中铜、锌的含量; 采用邻甲酚酞络合酮比色法测定血清钙含量; 采用酶联免疫吸附法测定DNA甲基转移酶(DNMT)1、DNMT3A、DNMT3B和组蛋白去乙酰化酶1(HDAC1)含量。从全部样本中随机抽取75%作为训练集(肺癌135例,肺良性疾病182例),以全部样本作为测试集,基于上述肿瘤标志物运用支持向量机建模。结果:肺癌组血清CEA、NSE、DNMT3A和DNMT3B含量均高于肺良性疾病组(P<0.05)。联合10项血清指标建立的支持向量机模型的准确度为90.3%,AUC为0.901(0.758~0.836),而Fisher判别模型则为79.7%和0.799(0.868~0.927)。结论:基于多项肿瘤标志物建立的支持向量机模型对肺癌预测效果较佳,应用到临床诊断更有价值。
Aim:To explore the value of support vector machine model based on tumor markers in the aided diagnosis of lung cancer.Methods:A total of 423 patients were collected, including 180 lung cancer patients and 243 patients with benign lung diseases. The levels of carcinoembryonic antigen(CEA), neuron-specific enolase(NSE)and gastrin in serum were detected by radioimmunoassay, the concentration of sialic acid was detected by the improved resorcinol chromogenic, the concentrations of serum copper and zinc were detected by inductively coupled plasma mass spectrometry,that of serum calcium,by O-cresol oxime complex ketone colorimetry, and the contents of DNA methyltransferase(DNMT)1, DNMT3A, DNMT3B and histone deacetylation enzyme 1(HDAC1)were determined by enzyme-linked immunosorbent assay. Among all the samples,75% were randomly selected as training set(135 cases of lung cancer, 182 cases of benign lung diseases), and all samples were used as the test set. Based on the above tumor markers, support vector machine model was developed.Results:The levels of CEA, NSE, DNMT3A and DNMT3B in the lung cancer group were higher than those of the lung benign diseases group(P<0.05). The accuracy of the support vector machine model based on the above tumor markers was 90.3%, and the AUC was 0.901(0.758-0.836).The accuracy of the Fisher discriminant analysis model was 79.7%,and the AUC was 0.799(0.868-0.927).Conclusion:The support vector machine model based on multi-tumor markers has good predictive effect on lung cancer and is more valuable for clinical diagnosis.


[1] BRAY F,FERLAY J,SOERJOMATARAM I,et al.Global cancer statistics 2018: GLOBOCAN estimates of incidence and mortality worldwide for 36 cancers in 185 countries[J].CA Cancer J Clin,2018,68(6):394
[2] CHEN W,ZHENG R,BAADE PD,et al.Cancer statistics in China, 2015[J].CA Cancer J Clin,2016,66(2):115
[3] 杨拴盈.肺癌早期诊断的现状、困惑和希望[J].西安交通大学学报(医学版),2011,32(1):1
[4] MIDTHUN DE.Screening for lung cancer[J].Clin Chest Med,2011,32(4):659
[5] 支修益,姜格宁.早期原发性肺癌诊断和治疗进展[J].中国医学前沿杂志:电子版,2015,7(2):1
[6] 赵威,李恩有,刘珊珊,等.肺癌诊断方法的比较与研究进展[J].现代生物医学进展,2016,16(9):1766
[7] OYA Y,YOSHIDA T,UEMURA T,et al.Serum ProGRP and NSE levels predicting small cell lung cancer transformation in a patient with ALK rearrangement-positive non-small cell lung cancer: a case report[J].Oncol Lett,2018,16(4):4219
[8] WANG JB,JIANG W,ZHANG T,et al.Increased CYFRA 21-1, CEA and NSE are prognostic of poor outcome for locally advanced squamous cell carcinoma in lung: a nomogram and recursive partitioning risk stratification analysis[J].Transl Oncol,2018,11(4):999
[9] MULEY T,ROLNY V,HE Y,et al.The combination of the blood based tumor biomarkers cytokeratin 19 fragments(CYFRA 21-1)and carcinoembryonic antigen(CEA)as a potential predictor of benefit from adjuvant chemotherapy in early stage squamous cell carcinoma of the lung(SCC)[J].Lung Cancer,2018,120:46
[10]YOON H,KIM N.Diagnosis and management of high risk group for gastric cancer[J].Gut Liver,2015,9(1):5
[11]SLAVIK T,ASSELAH F,FAKHRUDDIN N,et al.Diagnosis and predictive molecular analysis of non-small-cell lung cancer in the Africa-Middle East region: challenges and strategies for improvement[J].Clin Lung Cancer,2014,15(6):398
[12]DURUISSEAUX M,ESTELLER M.Lung cancer epigenetics: from knowledge to applications[J].Semin Cancer Biol,2018,51:116
[13]ENZ N,FRAGOSO F,GAMREKELI A,et al.Carcinoembryonic antigen-positive pleural effusion in early stage non-small cell lung cancer without pleural infiltration[J].J Thorac Dis,2018,10(5):E340
[14]ARRIETA O,VILLARREAL-GARZA C,MARTÍNEZ-BARRERA L,et al.Usefulness of serum carcinoembryonic antigen(CEA)in evaluating response to chemotherapy in patients with advanced non small-cell lung cancer: a prospective cohort study[J].BMC Cancer,2013,13:254
[15]GRUNNET M,SORENSEN JB.Carcinoembryonic antigen(CEA)as tumor marker in lung cancer[J].Lung Cancer,2012,76(2):138
[16]PIRINCCI N,GECIT I,GUNES M,et al.Levels of serum trace elements in renal cell carcinoma cases[J].Asian Pac J Cancer Prev,2013,14(1):499
[18]BÜLL C,STOEL MA,DEN BROK MH,et al.Sialic acids sweeten a tumor's life[J].Cancer Res,2014,74(12):3199
[19]TELL R,RIVERA CA,ESKRA J,et al.Gastrin-releasing peptide signaling alters colon cancer invasiveness via heterochromatin protein 1Hsβ[J].Am J Pathol,2011,178(2):672
[20]SONG LJ,LIU RJ,ZENG Z,et al.Gastrin inhibits a novel, pathological colon cancer signaling pathway involving EGR1, AE2, and P-ERK[J].J Mol Med(Berl),2012,90(6):707
[21]ZABLOCKA-SLOWINSKA K,PLACZKOWSKA S,PRESCHA A,et al.Serum and whole blood Zn, Cu and Mn profiles and their relation to redox status in lung cancer patients[J].J Trace Elem Med Biol,2018,45:78
[22]KIM J,SHIN H.Breast cancer survivability prediction using labeled,unlabeled,and pseudo-labeled patient data[J].J Am Med Inform Assoc,2013,20(4):613
[23]LEE T,JUNG WM,LEE IS,et al.Data mining of acupoint characteristics from the classical medical text: DongUiBoGam of Korean Medicine[J].Evid Based Complement Alternat Med,2014,2014:329563
[24]PARSAEI H,STASHUK DW.SVM-based validation of motor unit potential trains extracted by EMG signal decomposition[J].IEEE Trans Biomed Eng,2012,59(1):183
[25]CHANG SW,ABDUL-KAREEM S,MERICAN AF.Oral cancer prognosis based on clinicopathologic and genomic markers using a hybrid of feature selection and machine learning methods[J].BMC Bioinformatics,2013,14:170
[26]WEI D.A multigene support vector machine predictor for metastasis of cutaneous melanoma[J].Mol Med Rep,2018,17(2):2907


 LIU Hongyan),QIAO Yuhuan),YUAN Ling),et al.Variance mass spectrum comparison with cervical and esophageal squamous cell carcinoma[J].JOURNAL OF ZHENGZHOU UNIVERSITY(MEDICAL SCIENCES),2009,(03):241.
 LIU Tianzhou),ZHANG Lin),WANG Qingfang),et al.Diagnostic value of combined determination of serum CEA,NSE,SCCAg and CYFRA211 for lung cancer[J].JOURNAL OF ZHENGZHOU UNIVERSITY(MEDICAL SCIENCES),2010,(03):809.
 YAN Mingzhu),MA Zhiyong),ZHAO Yanqiu),et al.Effects of case management model on quality of life and self-perceived burden in lung cancer patients with chemotherapy[J].JOURNAL OF ZHENGZHOU UNIVERSITY(MEDICAL SCIENCES),2018,(03):241.[doi:10.13705/j.issn.1671-6825.2017.08.003]
 XU Yongchao,DING Mingcui,FENG Xiaolei,et al.Relationship of miR-210 gene expression and polymorphism to the lung cancer susceptibility[J].JOURNAL OF ZHENGZHOU UNIVERSITY(MEDICAL SCIENCES),2018,(03):726.[doi:10.13705/j.issn.1671-6825.2018.06.001]
 SONG Yongan,LIU Haiyang,ZHANG Xiaoju.Effects of apatinib on proliferation, ROS level and apoptosis of lung adenocarcinoma H1650 cells[J].JOURNAL OF ZHENGZHOU UNIVERSITY(MEDICAL SCIENCES),2019,(03):223.[doi:10.13705/j.issn.1671-6825.2018.09.011]
[6]江国强,方 芳.厄洛替尼联合康莱特对肺癌A549细胞增殖、侵袭及JAK2/STAT3信号通路的影响[J].郑州大学学报(医学版),2019,(03):418.[doi:10.13705/j.issn.1671-6825.2018.05.042]
 JIANG Guoqiang,FANG Fang.Effects of kanglaite and erlotinib combination on proliferation and invasion and JAK2/STAT3 signaling pathway of lung cancer A549 cells[J].JOURNAL OF ZHENGZHOU UNIVERSITY(MEDICAL SCIENCES),2019,(03):418.[doi:10.13705/j.issn.1671-6825.2018.05.042]
[7]邵 华,张 怡,黄 丽,等.NF1和p-ERK1/2蛋白在苯并芘联合脂多糖诱导的小鼠肺癌中的表达[J].郑州大学学报(医学版),2019,(06):835.[doi:10.13705/j.issn.1671-6825.2018.12.048]
 SHAO Hua,ZHANG Yi,HUANG Li,et al.Expressions of NF1 and p-ERK1/2 in B(a)p and LPS-induced lung cancer in mice[J].JOURNAL OF ZHENGZHOU UNIVERSITY(MEDICAL SCIENCES),2019,(03):835.[doi:10.13705/j.issn.1671-6825.2018.12.048]
[8]郑连喜),邓 超),何 平),等.上调叉头框转录因子J2对肺癌细胞凋亡及CHOP蛋白表达的影响[J].郑州大学学报(医学版),2019,(06):854.[doi:10.13705/j.issn.1671-6825.2018.08.045]
 ZHENG Lianxi),DENG Chao),HE Ping),et al.R734.2[J].JOURNAL OF ZHENGZHOU UNIVERSITY(MEDICAL SCIENCES),2019,(03):854.[doi:10.13705/j.issn.1671-6825.2018.08.045]
[9]郭 丹),闫秀明),杨 亮),等.HOXA6基因过表达对肺癌A549细胞增殖和凋亡的影响[J].郑州大学学报(医学版),2019,(06):909.[doi:10.13705/j.issn.1671-6825.2019.04.080]
 GUO Dan),YAN Xiuming),YANG Liang),et al.Effects of HOXA6 gene overexpression on proliferation and apoptosis of lung cancer A549 cells[J].JOURNAL OF ZHENGZHOU UNIVERSITY(MEDICAL SCIENCES),2019,(03):909.[doi:10.13705/j.issn.1671-6825.2019.04.080]


更新日期/Last Update: 2019-05-20