[1]吴 昊,林 庆,徐 全,等.ECT2-siRNA转染对肺腺癌A549细胞周期分布和凋亡的影响[J].郑州大学学报(医学版),2019,(03):406-409.[doi:10.13705/j.issn.1671-6825.2018.11.148]
 WU Hao,LIN Qing,XU Quan,et al.Effects of ECT2-siRNA transfection on cell cycle distribution and apoptosis of lung adenocarcinoma A549 cells[J].JOURNAL OF ZHENGZHOU UNIVERSITY(MEDICAL SCIENCES),2019,(03):406-409.[doi:10.13705/j.issn.1671-6825.2018.11.148]
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ECT2-siRNA转染对肺腺癌A549细胞周期分布和凋亡的影响()
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《郑州大学学报(医学版)》[ISSN:1671-6825/CN:41-1340/R]

卷:
期数:
2019年03期
页码:
406-409
栏目:
论著
出版日期:
2019-05-20

文章信息/Info

Title:
Effects of ECT2-siRNA transfection on cell cycle distribution and apoptosis of lung adenocarcinoma A549 cells
作者:
吴 昊林 庆徐 全柳阳春陈立如尹 随
江西省人民医院心胸外科 南昌 330000
Author(s):
WU HaoLIN QingXU QuanLIU YangchunCHEN LiruYIN Sui
Department of Cardiothoracic Surgery, Jiangxi People's Hospital, Nanchang 330000
关键词:
肺腺癌 ECT2 细胞周期 细胞凋亡
Keywords:
lung adenocarcinoma ECT2 cell cycle cell apoptosis
分类号:
R734.2
DOI:
10.13705/j.issn.1671-6825.2018.11.148
摘要:
目的:探讨ECT2-siRNA转染对肺腺癌A549细胞周期分布和凋亡的影响及其机制。方法:将体外培养的A549细胞分为空白组(未转染)、阴性对照组(转染阴性对照NC-siRNA)和干扰组(转染ECT2-siRNA),采用PI染色检测细胞周期分布,采用Annexin V-FITC和PI双染法检测凋亡,用Western blot检测细胞中ECT2、CyclinD1、P53和Cleaved Caspase-3蛋白的表达。结果:干扰组细胞中ECT2蛋白的表达水平低于空白组和阴性对照组(P<0.05)。与其他2组相比,干扰组S期和G2/M期细胞百分比降低,而G0/G1期细胞百分比和细胞凋亡率均升高(P<0.05),CyclinD1蛋白的表达水平降低,而P53和Cleaved Caspase-3蛋白的表达水平升高(P<0.05)。结论:靶向沉默ECT2的表达可诱导A549细胞周期阻滞和凋亡,其作用机制可能与下调CyclinD1,上调P53、Cleaved Caspase-3蛋白的表达有关。
Abstract:
Aim:To investigate the effects of ECT2-siRNA transfection on cell cycle distribution and apoptosis of lung adenocarcinoma A549 cells.Methods::A549 cells cultured in vitro were divided into blank group(untransfected), negative control group(transfected with negative control NC-siRNA)and interference group(transfected with ECT2-siRNA). The cell cycle distribution and apoptosis were detected by PI staining and Annexin V-FITC and PI staining,respectively. The expressions of ECT2, CyclinD1, P53 and Cleaved Caspase-3 protein were detected by Western blot.Results:The expression of ECT2 protein in the interference group was significantly lower than those in the blank group and negative control group(P<0.05). Compared with the other 2 groups, the percentage of cells at S phase and G2/M phase decreased significantly in the interference group, while the percentage of cells at G0/G1 phase and apoptotic rate increased significantly(P<0.05); at the same time, the expression level of CyclinD1 protein in the interference group was significantly lower, while the expression levels of P53 and Cleaved Caspase-3 protein were higher(P<0.05).Conclusion:ECT2-siRNA transfection could induce cell cycle arrest and apoptosis in A549 cells, and its mechanism may be related to down-regulation of CyclinD1 and up-regulation of P53 and Cleaved Caspase-3 protein expression.

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备注/Memo

备注/Memo:
【基金项目】江西省卫生厅课题资助项目(20151017)
【作者简介】徐全,通信作者,男,1972年10月生,硕士,主任医师,研究方向:肺癌、食管癌的防治,E-mail:xuquan7219@163.com
更新日期/Last Update: 2019-05-20