[1]江国强,方 芳.厄洛替尼联合康莱特对肺癌A549细胞增殖、侵袭及JAK2/STAT3信号通路的影响[J].郑州大学学报(医学版),2019,(03):418-422.[doi:10.13705/j.issn.1671-6825.2018.05.042]
 JIANG Guoqiang,FANG Fang.Effects of kanglaite and erlotinib combination on proliferation and invasion and JAK2/STAT3 signaling pathway of lung cancer A549 cells[J].JOURNAL OF ZHENGZHOU UNIVERSITY(MEDICAL SCIENCES),2019,(03):418-422.[doi:10.13705/j.issn.1671-6825.2018.05.042]
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厄洛替尼联合康莱特对肺癌A549细胞增殖、侵袭及JAK2/STAT3信号通路的影响()
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《郑州大学学报(医学版)》[ISSN:1671-6825/CN:41-1340/R]

卷:
期数:
2019年03期
页码:
418-422
栏目:
应用研究
出版日期:
2019-05-20

文章信息/Info

Title:
Effects of kanglaite and erlotinib combination on proliferation and invasion and JAK2/STAT3 signaling pathway of lung cancer A549 cells
作者:
江国强方 芳
武警四川省总队医院呼吸科 四川乐山 614000
Author(s):
JIANG GuoqiangFANG Fang
Department of Respiration, Sichuan General Team Hospital of Armed Police Force, Leshan, Sichuan 614000
关键词:
厄洛替尼 康莱特 肺癌 A549细胞 增殖 侵袭 JAK2/STAT3信号通路
Keywords:
erlotinib kanglaite lung cancer A549 cell proliferation invasion JAK2/STAT3 signaling pathway
分类号:
R734.2
DOI:
10.13705/j.issn.1671-6825.2018.05.042
摘要:
目的:探讨厄洛替尼和康莱特联用对肺癌A549细胞增殖、侵袭及JAK2/STAT3信号通路的影响。方法:肺癌A549细胞分为对照组(细胞不经特殊处理)、厄洛替尼组(10 mg/L厄洛替尼处理细胞48 h)、康莱特组(20 mg/L康莱特处理细胞48 h)和联合组(10 mg/L厄洛替尼和20 mg/L康莱特共同处理细胞48 h)。采用MTT法及Transwell小室分别检测4组细胞的活力及侵袭能力,采用Western blot检测4组细胞中增殖相关蛋白(Ki-67)、侵袭相关蛋白[E-钙黏附蛋白(E-cadherin)和基质蛋白酶2(MMP-2)]及JAK2/STAT3信号通路蛋白[JAK2、磷酸化JAK2(p-JAK2)、STAT3和磷酸化STAT3(p-STAT3)]的表达。结果:厄洛替尼组和康莱特组细胞活力、侵袭能力及Ki-67、MMP-2、p-JAK2和p-STAT3蛋白表达均低于对照组,E-cadherin蛋白表达高于对照组(P<0.05),联合组细胞活力、侵袭能力及Ki-67、MMP-2、p-JAK2和p-STAT3蛋白的表达均低于厄洛替尼组和康莱特组,E-cadherin蛋白的表达高于厄洛替尼组和康莱特组(P<0.05)。结论:厄洛替尼和康莱特能抑制肺癌细胞活力及侵袭能力,其机制与抑制JAK2/STAT3信号通路有关。
Abstract:
Aim:To investigate the effects of erlotinib and kanglaite combination on the proliferation and invasion and JAK2/STAT3 signaling pathway of lung cancer A549 cells.Methods:A549 cells were divided into control group(cells without special treatment), erlotinib group(10 mg/L erlotinib treated cells for 48 hour), kanglaite group(20 mg/L kanglaite treated cells for 48 hour)and combination group(10 mg/L Erlotinib and 20 mg/L Kanglaite treated cells), cell viability and invasiveness were detected by MTT and Transwell chamber; the expressions of Ki-67, E-cadherin, MMP-2, JAK2, p-JAK2, STAT3 and p-STAT3 protein were detected by Western blot.Results:Cell viability, cell invasion and Ki-67, MMP-2, p-JAK2 and p-STAT3 protein expressions in erlotinib group and kanglaite group were significantly lower than those of the control group, while E-cadherin protein expression was significantly higher than the control group(P<0.05); cell viability, cell invasion and Ki-67, MMP-2, p-JAK2 and p-STAT3 protein expressions in combination group were significantly lower than those in erlotinib group and kanglaite group,while the expression of E-cadherin protein was significantly higher than those in erlotinib group and kanglaite group(P<0.05).Conclusion:Erlotinib and kanglaite can inhibit lung cancer cell activity and invasion, the two combined effect stronger, and the mechanism is related to the inhibition of JAK2/STAT3 signaling pathway.

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备注/Memo

备注/Memo:
【基金项目】四川省医学科研青年创新课题计划项目(Q16015)
【作者简介】江国强,男,1966年4月生,本科,主任医师,研究方向:呼吸系统疾病的诊治,E-mail:guoqiang2726@126.com
更新日期/Last Update: 2019-05-20