[1]张 茂,孙浩言,张 磊.降低miR-21表达对顺铂耐药A549细胞药物敏感性的影响[J].郑州大学学报(医学版),2019,(03):422-425.[doi:10.13705/j.issn.1671-6825.2018.05.124]
 ZHANG Mao,SUN Haoyan,ZHANG Lei.Inhibition of miR-21 enhances sensitivity of cisplatin-resistant A549 cells to cisplatin[J].JOURNAL OF ZHENGZHOU UNIVERSITY(MEDICAL SCIENCES),2019,(03):422-425.[doi:10.13705/j.issn.1671-6825.2018.05.124]
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降低miR-21表达对顺铂耐药A549细胞药物敏感性的影响()
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《郑州大学学报(医学版)》[ISSN:1671-6825/CN:41-1340/R]

卷:
期数:
2019年03期
页码:
422-425
栏目:
应用研究
出版日期:
2019-05-20

文章信息/Info

Title:
Inhibition of miR-21 enhances sensitivity of cisplatin-resistant A549 cells to cisplatin
作者:
张 茂孙浩言张 磊
宜宾市第一人民医院药剂科 四川宜宾 644000
Author(s):
ZHANG MaoSUN HaoyanZHANG Lei
Department of Pharmacy,the First People's Hospital of Yibin City, Yibin, Sichuan 644000
关键词:
miR-21 非小细胞肺癌 顺铂 敏感性
Keywords:
miR-21 non-small cell lung cancer cisplatin sensitivity
分类号:
R734.2
DOI:
10.13705/j.issn.1671-6825.2018.05.124
摘要:
目的:探讨降低miR-21表达对顺铂(DDP)耐药A549细胞药物敏感性的影响。方法:采用qRT-PCR对顺铂敏感和耐药的NSCLC患者癌组织、A549及顺铂耐药A549(A549/DDP)细胞中miR-21的表达水平进行检测。利用脂质体转染法将miR-21抑制剂(anti-miR-21)、阴性对照(anti-miR-NC)转染到A549/DDP中,检测顺铂对细胞的IC50,MTT法检测细胞增殖活性,Annexin V-FITC/PI双染法检测细胞凋亡,分光光度法检测Caspase-3活性。结果:与顺铂敏感肿瘤组织和A549细胞相比,顺铂耐药肿瘤组织和A549/DDP中miR-21表达水平升高(P<0.05)。与anti-miR-NC转染的A549/DDP细胞比较,顺铂对anti-miR-21转染的A549/DDP细胞IC50和细胞增殖活性降低(P<0.05),细胞凋亡率和Caspase-3活性升高(P<0.05)。结论:抑制miR-21可提高肺癌细胞对顺铂的敏感性。
Abstract:
Aim:To investigate the effects of inhibiting miR-21 on the sensitivity of cisplatin(DDP)-resistant A549 cells to DDP and its mechanism.Methods:qRT-PCR was used to detect the expression of miR-21 in sensitive or insensitive lung cancer tissue and A549 cells. The inhibitor of miR-21(anti-miR-21), miRNA inhibitor negative control(anti-miR-NC)were transfected into DDP-resistant lung cancer cells(A549/DDP)under the action of Lipofectamine 2000. IC50 of DDP to the cells was detected.Cell proliferation activity and apoptosis were measured by MTT and Annexin V-FITC/PI staining,respectively, and Caspase-3 activity was determined by spectrophotometric method.Results:The expression of miR-21 in DDP-resistant tumor tissue or A549/DDP was significantly higher than that in DDP-sensitive tumor tissue or 549 cells(P<0.05). The IC50 of DDP to anti-miR-21 transfection A549/DDP cells and proliferation activity were lower,while the apoptosis rate and Caspase-3 activity in anti-miR-21 transfection group were higher than those in anti-miR-NC transfection group(P<0.05).Conclusion: Inhibition of miR-21 enhances the sensitivity of lung cancer cells to DDP.

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备注/Memo

备注/Memo:
【基金项目】四川省科技攻关基金资助项目(2017JY0315)
【作者简介】张茂,通信作者,男,1972年 2月生,副主任药师,研究方向:医院药学,E-mail:zhangmao26@163.com
更新日期/Last Update: 2019-05-20