[1]董良杰),王勤俭),王单一),等.白芍总苷对人骨关节炎软骨细胞增殖及p38MAPK蛋白表达的影响[J].郑州大学学报(医学版),2019,(03):431-435.[doi:10.13705/j.issn.1671-6825.2019.02.011]
 DONG Liangjie),WANG Qinjian),WANG Danyi),et al.Effects of total glucosides of paeonia on proliferation of osteoarthritic chondrocytes by regulating p38MAPK[J].JOURNAL OF ZHENGZHOU UNIVERSITY(MEDICAL SCIENCES),2019,(03):431-435.[doi:10.13705/j.issn.1671-6825.2019.02.011]
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白芍总苷对人骨关节炎软骨细胞增殖及p38MAPK蛋白表达的影响()
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《郑州大学学报(医学版)》[ISSN:1671-6825/CN:41-1340/R]

卷:
期数:
2019年03期
页码:
431-435
栏目:
应用研究
出版日期:
2019-05-20

文章信息/Info

Title:
Effects of total glucosides of paeonia on proliferation of osteoarthritic chondrocytes by regulating p38MAPK
作者:
董良杰1) 王勤俭1)王单一2)释延医3)释延无3)
1)河南省中医院(河南中医药大学第二附属医院)骨伤病诊疗中心骨二科 郑州 450000 2)河南中医药大学针灸推拿学院 郑州 450008 3)少林药局 河南登封 452400
Author(s):
DONG Liangjie1)WANG Qinjian1)WANG Danyi2)SHI Yanyi3)SHI Yanwu3)
1)Second Department of Orthopaedics,Bone Injury Diagnosis and Treatment Center,Henan Traditional Chinese Medicine Hospital(the Second Affiliated Hospital,Henan University of Traditional Chinese Medicine),Zhengzhou 450000 2)College of Acupuncture and Massage,Henan University of Traditional Chinese Medicine,Zhengzhou 450008 3)Shaolin Medicine Bureau,Dengfeng,Henan 452400
关键词:
白芍总苷 p38MAPK信号通路 骨关节炎 软骨细胞 增殖
Keywords:
total glucosides of paeonia p38MAPK signaling pathway osteoarthritis chondrocyte proliferation
分类号:
R684
DOI:
10.13705/j.issn.1671-6825.2019.02.011
摘要:
目的:研究白芍总苷对人骨关节炎软骨细胞增殖和p38MAPK表达的影响。方法:分离膝骨关节炎患者的关节软骨细胞,分为4组,对照组只加培养基,白芍总苷组加入白芍总苷终浓度为8 μmol/L的培养基,激动剂组加入p38MAPK信号通路激动剂anisomycin终浓度为2 μg/L的培养基,联合组加入含白芍总苷和anisomycin的培养基(终浓度同前)。分组培养24、48、72 h后,MTT法检测细胞增殖能力; 分组培养48 h后,碘化丙啶单染法检测细胞周期,qRT-PCR法检测细胞中Ki67、PCNA mRNA表达水平,Western blot法检测细胞中p38MAPK和磷酸化p38MAPK(p-p38MAPK)蛋白表达水平。结果:Anisomycin单独作用可抑制骨关节炎软骨细胞的增殖,阻滞细胞周期进展,促进p38MAPK和p-p38MAPK蛋白的表达(P<0.05); 白芍总苷单独作用则促进细胞增殖和细胞周期进展,抑制p38MAPK和p-p38MAPK蛋白的表达(P<0.05); 两者联用,白芍总苷可以拮抗anisomycin诱导的骨关节炎软骨细胞p38MAPK和p-p38MAPK的表达增加和增殖抑制作用(P<0.05)。结论:白芍总苷可能通过抑制p38MAPK信号通路,促进人骨关节炎软骨细胞的增殖。
Abstract:
Aim:To study the effects of total glucosides of paeonia(TGP)on the proliferation and p38MAPK protein expression of human osteoarthritic chondrocytes.Methods:Human osteoarthritic chondrocytes were isolated from the patients with knee osteoarthritis, and were allocated into 4 groups,TGP group(cultured with 8 μmol/L TGP), agonist group(cultured with 2 μg/L anisomycin which was p38MAPK signal pathway agonist), combination group(cultured with 8 μmol/L TGP and 2 μg/L anisomycin), blank control group(cultured without TGP or anisomycin). After 24,48,72 hour culture,cell proliferation was detected by MTT assay. After 48 hour culture, the cell cycle was detected by PI monochrome staining,the expressions of Ki67 and PCNA mRNA was detected by qRT-PCR, and the p38MAPK and phosphorylated-p38MAPK(p-p38MAPK)proteins were detected by Western blot.Results:When treated with anisomycin,the proliferation ability of human osteoarthritic chondrocytes was decreased, the cell cycle was blocked at G0/G1 phase,and the expression levels of p38MAPK and p-p38MAPK were increased(P<0.05). When treated with TGP,the proliferation ability of human osteoarthritic chondrocytes was increased, the cell cycle was progressing,and the expression levels of p38MAPK and p-p38MAPK were decreased(P<0.05). The combination of the two drugs antagonized each other(P<0.05).Conclusion:TGP may promote the proliferation of human osteoarthritic chondrocytes by inhibiting p38MAPK signaling pathway.

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备注/Memo

备注/Memo:
【基金项目】 河南省科技攻关计划项目(162102310446)
【作者简介】 董良杰,男,1976年5月生,副主任医师,研究方向:中医骨伤,E-mail:dongliangjie915@163.com
更新日期/Last Update: 2019-05-20