[1]郭洪耀),乔军波),林 斌),等.DKK3基因过表达对人瘢痕疙瘩成纤维细胞增殖和凋亡的影响[J].郑州大学学报(医学版),2019,(03):474-477.[doi:10.13705/j.issn.1671-6825.2018.09.120]
 GUO Hongyao),QIAO Junbo),LIN Bin),et al.Effects of overexpression of DKK3 gene on proliferation and apoptosis of keloid fibroblasts[J].JOURNAL OF ZHENGZHOU UNIVERSITY(MEDICAL SCIENCES),2019,(03):474-477.[doi:10.13705/j.issn.1671-6825.2018.09.120]
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DKK3基因过表达对人瘢痕疙瘩成纤维细胞增殖和凋亡的影响()
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《郑州大学学报(医学版)》[ISSN:1671-6825/CN:41-1340/R]

卷:
期数:
2019年03期
页码:
474-477
栏目:
应用研究
出版日期:
2019-05-20

文章信息/Info

Title:
Effects of overexpression of DKK3 gene on proliferation and apoptosis of keloid fibroblasts
作者:
郭洪耀1)乔军波2)林 斌1)沙树奎1)
1)南阳市中医院烧伤整形科 河南南阳 473000 2)郑州大学第三附属医院血管瘤外科 郑州 450052
Author(s):
GUO Hongyao1)QIAO Junbo2)LIN Bin1)SHA Shukui1)
1)Department of Burn and Plastic Surgery,Nanyang Hospital of Traditional Chinese Medicine,Nanyang,Henan 473000 2)Department of Hemangioma Surgery, the Third Affiliated Hospital,Zhengzhou University,Zhengzhou 450052
关键词:
DKK3 瘢痕 成纤维细胞 细胞增殖 细胞凋亡
Keywords:
DKK3 scar fibroblast cell proliferation cell apoptosis
分类号:
R622
DOI:
10.13705/j.issn.1671-6825.2018.09.120
摘要:
目的:探究DKK3基因过表达对人瘢痕成纤维细胞增殖和凋亡的影响。方法:取瘢痕疙瘩组织21例和正常皮肤组织20例,采用qRT-PCR检测其中DKK3 mRNA的表达水平。构建真核表达质粒pcDNA3.0-DKK3,通过脂质体介导的方法将其转入瘢痕成纤维细胞中(DKK3组),未转染细胞为对照组,转染pcDNA3.0空质粒的细胞为pcDNA组。转染48 h后,qRT-PCR和Western blot检测转染效率; CCK-8法检测成纤维细胞的增殖情况; 双染法检测成纤维细胞的凋亡情况。结果:DKK3 mRNA在瘢痕疙瘩组织中表达下调(P<0.05)。3组瘢痕疙瘩成纤维细胞中DKK3 mRNA和蛋白的表达差异有统计学意义(P<0.05),DKK3组高于其他两组(P<0.05)。3组细胞凋亡和增殖情况差异有统计学意义(P<0.05),DKK3组增殖减少,凋亡增加(P<0.05)。结论:DKK3基因过表达可抑制瘢痕疙瘩成纤维细胞的增殖并诱导其发生凋亡。
Abstract:
Aim:To investigate the effects of overexpression of DKK3 gene on proliferation and apoptosis of human keloid fibroblasts.Methods:A total of 21 cases of keloid tissue and 20 cases of normal skin tissue were taken. qRT-PCR was used to detect the expression level of DKK3 mRNA.The eukaryotic expression plasmid pcDNA3.0-DKK3 was constructed and transferred into scar fibroblasts by liposome-mediated method(DKK3 group), untransfected cells were used as control group, and empty plasmid pcDNA3.0 was transfected as negative control(pcDNA group).After transfection for 48 hours, qRT-PCR and Western blot were used to detect the transfection efficiency; the proliferation of fibroblasts was detected by CCK-8 method; the apoptosis of fibroblasts was detected by double staining method.Results:The level of DKK3 mRNA was down-regulated in keloid tissue(P<0.05). The levels of DKK3 mRNA and protein in keloid fibroblasts of the three groups were significantly different(P<0.05),which was higher in the DKK3 group than those in the other two groups(P<0.05).The apoptosis and proliferation among the 3 groups were statistically different(P<0.05), the proliferation of keloid fibroblasts in DKK3 group was decreased(P<0.05), and the apoptosis was increased(P<0.05).Conclusion:Overexpression of DKK3 gene could inhibit proliferation and induce apoptosis of keloid fibroblasts.

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备注/Memo

备注/Memo:
【基金项目】河南省自然科学基金项目(0611040400)
【作者简介】郭洪耀,通信作者,男,1973年4月生,硕士,副主任医师,研究方向:烧伤整形,E-mail:ghyghy212@126.com
更新日期/Last Update: 2019-05-20