[1]张璐玉,崔艳艳,王文杰,等.p97抑制剂NMS-873对Eca109细胞增殖、凋亡及迁移能力的影响[J].郑州大学学报(医学版),2019,(04):489-492.[doi:10.13705/j.issn.1671-6825.2018.10.051]
 ZHANG Luyu,CUI Yanyan,WANG Wenjie,et al.Effects of p97 inhibitor NMS-873 on proliferation,apoptosis and migration of Eca109 cells[J].JOURNAL OF ZHENGZHOU UNIVERSITY(MEDICAL SCIENCES),2019,(04):489-492.[doi:10.13705/j.issn.1671-6825.2018.10.051]
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p97抑制剂NMS-873对Eca109细胞增殖、凋亡及迁移能力的影响()
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《郑州大学学报(医学版)》[ISSN:1671-6825/CN:41-1340/R]

卷:
期数:
2019年04期
页码:
489-492
栏目:
食管癌研究
出版日期:
2019-07-20

文章信息/Info

Title:
Effects of p97 inhibitor NMS-873 on proliferation,apoptosis and migration of Eca109 cells
作者:
张璐玉崔艳艳王文杰张彦婷刘艳霞马珊珊刘红涛张 乐刘腾飞周建康王亚苹关方霞
郑州大学生命科学学院 郑州 450001
Author(s):
ZHANG Luyu CUI Yanyan WANG Wenjie ZHANG Yanting LIU Yanxia MA Shanshan LIU Hongtao ZHANG Le LIU Tengfei ZHOU Jiankang WANG Yaping GUAN Fangxia
College of Life Sciences, Zhengzhou University, Zhengzhou 450001
关键词:
食管鳞状细胞癌 p97 NMS-873 Eca109细胞
Keywords:
esophageal squamous cell carcinoma p97 NMS-873 Eca109 cell
分类号:
R735.1
DOI:
10.13705/j.issn.1671-6825.2018.10.051
摘要:
目的:探讨p97抑制剂NMS-873对食管鳞状细胞癌Eca109细胞增殖、凋亡、迁移的影响。方法:用不同浓度的NMS-873分别处理Eca109细胞24、48、72 h,采用CCK-8法检测细胞增殖; 在此基础上选用1、2、3 μmol/L NMS-873分别作用于Eca109细胞48 h,采用AnnexinV-FITC/PI双染法检测细胞凋亡,Transwell迁移实验检测细胞迁移能力的变化,EdU荧光法检测细胞增殖。结果:NMS-873可呈时间和剂量依赖性抑制Eca109细胞增殖; 1、2、3 μmol/L的NMS-873分别作用于Eca109细胞48 h后,与阴性对照组相比,随着NMS-873浓度的增加,NMS-873组Eca109细胞凋亡率提高,迁移细胞数减少,增殖率降低(P<0.05)。结论:NMS-873能有效抑制Eca109细胞增殖,促进细胞凋亡,并降低细胞迁移能力。
Abstract:
Aim:To investigate the effects of p97 inhibitor NMS-873 on proliferation, apoptosis and migration of esophageal squamous cell carcinoma cell line Eca109.Methods:Eca109 cells were treated with different concentrations of NMS-873 for 24, 48, and 72 hours, respectively. CCK-8 assay was used to detect proliferation of Eca109 cells. Based on this, 1,2 and 3 μmol/L NMS-873 were chosen and treated Eca109 cells for 48 hours. AnnexinV-FITC/PI double staining was used to detect apoptosis,Transwell migration assay was used to detect cell migration ability, and EdU fluorescence assay was used to detect cell proliferation.Results:CCK-8 results showed that NMS-873 significantly inhibited the proliferation of Eca109 cells in a time- and dose-dependent manner(P<0.05). Subsequently, after NMS-873 at 1,2,and 3 μmol/L being used to treat Eca109 cells for 48 hours,compared with the negative control group, with the increase of NMS-873 concentration, the apoptosis rate of Eca109 cells in the NMS-873 groups was significantly increased,while the number of migrated cells and the proliferation rate were significantly decreased(P<0.05).Conclusion:NMS-873 can effectively inhibit the proliferation of Eca109 cells, promote cell apoptosis and reduce cell migration capability.

参考文献/References:

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备注/Memo

备注/Memo:
【基金项目】国家自然科学基金资助项目(81471306); 河南省高校科技创新团队项目(15IRTSTHN022); 河南省自然科学基金资助项目(182300410377); 河南省高等学校重点科研项目计划(17A180016)
【作者简介】关方霞,通信作者,女,1969年2月生,博士,教授,研究方向:干细胞和生物医学,E-mail:guanfangxia@126.com; 张彦婷,通信作者,女,1985年11月生,博士,讲师,研究方向:肿瘤生物学,E-mail:zhangyt@zzu.edu.cn
更新日期/Last Update: 2019-07-20