[1]宋 歌),刘萍萍),李 静),等.重症肌无力患者胸腺组织中差异表达信号通路分子的筛查[J].郑州大学学报(医学版),2019,(04):527-530.[doi:10.13705/j.issn.1671-6825.2018.10.032]
 SONG Ge),LIU Pingping),LI Jing),et al.Screening of differentially expressed signal pathway molecules in thymic tissue of myasthenia gravis patients[J].JOURNAL OF ZHENGZHOU UNIVERSITY(MEDICAL SCIENCES),2019,(04):527-530.[doi:10.13705/j.issn.1671-6825.2018.10.032]
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重症肌无力患者胸腺组织中差异表达信号通路分子的筛查()
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《郑州大学学报(医学版)》[ISSN:1671-6825/CN:41-1340/R]

卷:
期数:
2019年04期
页码:
527-530
栏目:
论著
出版日期:
2019-07-20

文章信息/Info

Title:
Screening of differentially expressed signal pathway molecules in thymic tissue of myasthenia gravis patients
作者:
宋 歌12) 刘萍萍1)李 静1)李倩如1)高 峰3)张清勇4)崔新征4)杜 英1)
1)郑州大学基础医学院免疫学系 郑州 450001;2)河南省红十字血液中心 郑州 450008;3)郑州大学医药科学研究院 郑州 450052;4)河南省人民医院重症肌无力综合诊疗中心 郑州 450003
Author(s):
SONG Ge12)LIU Pingping1)LI Jing1)LI Qianru1)GAO Feng3)ZHANG Qingyong4)CUI Xinzheng4)DU Ying1)
1)Department of Immunology, Basic Medical College, Zhengzhou University,Zhengzhou 450001;2)Henan Red Cross Blood Center,Zhengzhou 450008;3)Institute of Medical and Pharmaceutical Sciences, Zhengzhou University,Zhengzhou 450052;4)Myasthenia Gravis Comprehensive Diagnosis and Treatment Center, Henan Provincial People's Hospital,Zhengzhou 450003
关键词:
信号通路 重症肌无力 胸腺
Keywords:
signal transduction myasthenia gravis thymus
分类号:
R392.1
DOI:
10.13705/j.issn.1671-6825.2018.10.032
摘要:
目的:探讨差异表达信号通路分子在重症肌无力(MG)患者胸腺组织中的表达及与疾病发生的关系。方法:取胸腺组织增生的MG患者及对照胸腺组织,提取总RNA,利用人信号转导通路发现者基因芯片检测2组的差异表达基因,同时选取芯片中的3个基因进行qRT-PCR验证。结果:基因芯片检测结果显示,MG组与对照组胸腺组织差异表达基因有22种,其中SOCS3、IRF1、HES1、FOSL1、EMP1、GADD45B、ADM、SERPINE1等共18个基因mRNA的表达在MG组增高,4个降低,qRT-PCR验证结果与芯片结果一致。结论:MG患者胸腺组织多条信号通路分子表达异常,可能与MG的发生有关。
Abstract:
Aim:To investigate the differentially expressed signal pathway molecules in thymic tissue of patients with myasthenia gravis(MG)and their relationship with the pathological progress.Methods:The total RNA from thymic tissue of MG patients and controls was extracted using Trizol reagent. The gene chip of human signal transduction pathway finder was used. The differences of gene expression between MG group and control group were compared. Among the different genes,three genes were selected for validation by qRT-PCR.Results:Gene chip results showed that there were 22 differentially expressed genes in thymus tissue between MG patients and controls, the mRNA expressions of 18 genes(SOCS3,IRF1,HES1,FOSL1,EMP1,GADD45B,ADM,SERPINE1,etc)were significantly increased in MG patients,and 4 genes were decreased. The results of qRT-PCR were consistent with those of the gene chip.Conclusions:The abnormal expression of multiple signal pathway molecules in thymic tissue of MG patients involves some kinds of signaling pathways,which may be associated with the pathogenesis of the disease.

参考文献/References:

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备注/Memo

备注/Memo:
【基金项目】国家自然科学基金资助项目(81471545)
【作者简介】 杜英,通信作者,女,1962年9月生,博士,教授,研究方向:免疫细胞异常分化与自身免疫病,E-mail:duying@zzu.edu.cn
更新日期/Last Update: 2019-07-20