[1]贺丽娜,胡杨志,周 轩,等.沉默PROX1表达对胃癌AGS细胞增殖和侵袭能力的影响[J].郑州大学学报(医学版),2019,(04):559-563.[doi:10.13705/j.issn.1671-6825.2018.08.151]
 HE Lina,HU Yangzhi,ZHOU Xuan,et al.Effects of silencing PROX1 expression on proliferation and invasion of gastric cancer AGS cells[J].JOURNAL OF ZHENGZHOU UNIVERSITY(MEDICAL SCIENCES),2019,(04):559-563.[doi:10.13705/j.issn.1671-6825.2018.08.151]
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沉默PROX1表达对胃癌AGS细胞增殖和侵袭能力的影响()
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《郑州大学学报(医学版)》[ISSN:1671-6825/CN:41-1340/R]

卷:
期数:
2019年04期
页码:
559-563
栏目:
应用研究
出版日期:
2019-07-20

文章信息/Info

Title:
Effects of silencing PROX1 expression on proliferation and invasion of gastric cancer AGS cells
作者:
贺丽娜胡杨志周 轩胡志立
湘南学院附属医院胃肠外科 湖南郴州 423000
Author(s):
HE LinaHU YangzhiZHOU XuanHU Zhili
Department of Gastrointestinal Surgery, the Affiliated Hospital, Xiangnan University, Chenzhou, Hunan 423000
关键词:
PROX1 胃癌 侵袭 迁移 AGS细胞
Keywords:
PROX1 gastric cancer invasion migration AGS cell
分类号:
R735.2
DOI:
10.13705/j.issn.1671-6825.2018.08.151
摘要:
目的:探讨沉默PROX1表达对胃癌细胞增殖和侵袭能力的影响。方法:胃癌AGS细胞分别感染PROX1 siRNA和阴性对照慢病毒(设置为干扰组和阴性对照组),以没有感染慢病毒的AGS细胞为空白对照组。采用qRT-PCR和Western blot法检测细胞中PROX1 mRNA和蛋白的表达,MTT方法测定细胞增殖情况,Transwell小室检测细胞侵袭和迁移能力,Western blot检测细胞中上皮间质转化(EMT)相关蛋白上皮性钙黏附素(E-cadherin)、波形蛋白(Vimentin)和侵袭迁移相关蛋白基质金属蛋白酶-2(MMP-2)、基质金属蛋白酶-9(MMP-9)表达水平。结果:干扰组细胞中PROX1 mRNA和蛋白表达水平均低于阴性对照组和空白对照组(P<0.05)。与阴性对照组和空白对照组比较,干扰组细胞增殖能力降低,细胞侵袭和迁移数目减少,细胞中E-cadherin蛋白表达水平升高,Vimentin、MMP-2、MMP-9蛋白表达水平降低(P<0.05)。结论:沉默PROX1表达可抑制胃癌细胞增殖、侵袭和EMT。
Abstract:
Aim:To investigate the effects of siRNA silencing PROX1 expression on proliferation and invasion of gastric cancer cells.Methods:PROX1 siRNA lentiviral vector and negative control lentiviral vector were transfected into gastric cancer AGS cells, which were set as interference group and negative control group,and AGS cells without lentiviral infection were taken as blank control group. qRT-PCR and Western blot were used to detect the interference effect,cell proliferation was detected by MTT method, Transwell matrix assay was used to detect cell invasion and migration,and Western blot was used to detect the expression levels of E-cadherin, Vimentin, MMP-2 and MMP-9.Results:The levels of PROX1 mRNA and protein in the interference group were significantly lower than those in the negative control group and the blank control group(P<0.05).Compared with the negative control group and the blank control group,the cell proliferation ability of the interference group was reduced, the numbers of cell invasion and migration also decreased, the level of E-cadherin protein increased, and the levels of Vimentin, MMP-2 and MMP-9 decreased(P<0.05).Conclusion:Silencing PROX1 expression could inhibit proliferation, invasion and EMT of gastric cancer cells.

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备注/Memo

备注/Memo:
【基金项目】湖南省自然科学基金资助项目(13JJ6062)
【作者简介】胡志立,通信作者,男,1978年3月生,硕士,副主任医师,研究方向:胃肠道恶性肿瘤的基础和临床,E-mail:50381234@qq.com
更新日期/Last Update: 2019-07-20