[1]方兴根),盛 斌).miR-577对脑胶质瘤U251细胞活力和凋亡的影响[J].郑州大学学报(医学版),2019,(04):571-574.[doi:10.13705/j.issn.1671-6825.2018.11.119]
 FANG Xinggen),SHENG Bin).Effects of miR-577 on viability and apoptosis of glioma cells U251[J].JOURNAL OF ZHENGZHOU UNIVERSITY(MEDICAL SCIENCES),2019,(04):571-574.[doi:10.13705/j.issn.1671-6825.2018.11.119]
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miR-577对脑胶质瘤U251细胞活力和凋亡的影响()
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《郑州大学学报(医学版)》[ISSN:1671-6825/CN:41-1340/R]

卷:
期数:
2019年04期
页码:
571-574
栏目:
应用研究
出版日期:
2019-07-20

文章信息/Info

Title:
Effects of miR-577 on viability and apoptosis of glioma cells U251
作者:
方兴根1)盛 斌2)
1)皖南医学院弋矶山医院神经外科 安徽芜湖 241001;2)皖南医学院第二附属医院神经外科 安徽芜湖 241001
Author(s):
FANG Xinggen1)SHENG Bin2)
1)Department of Neurosurgery, Yijishan Hospital, Wannan Medical College, Wuhu, Anhui 241001;2)Department of Neurosurgery, the Second Affiliated Hospital, Wannan Medical College, Wuhu, Anhui 241001
关键词:
脑胶质瘤 miR-577 Wnt/β-catenin通路 凋亡 U251细胞
Keywords:
glioma miR-577 Wnt/β-catenin pathway apoptosis U251 cell
分类号:
R739.41
DOI:
10.13705/j.issn.1671-6825.2018.11.119
摘要:
目的:探讨miR-577调节Wnt/β-catenin信号通路蛋白Wnt2b对脑胶质瘤U251细胞活力和凋亡的影响。方法:将U251细胞分为miR-对照组(转染miR-577阴性对照)、miR-577组(转染miR-577模拟物)、抑制对照组(转染miR-577抑制物对照)、miR-577抑制组(转染miR-577抑制物)、miR-577+Wnt2b组(转染miR-577模拟物与pcDNA-Wnt2b),采用qRT-PCR法检测转染后细胞miR-577的表达,CCK-8法及流式细胞术分别检测细胞活力和凋亡率。通过双荧光报告基因检测系统验证miR-577和Wnt2b的靶向关系。结果:miR-577组与miR-对照组相比,miR-577的表达水平升高。双荧光报告基因证实Wnt2b是miR-577的靶基因,miR-577组与miR-对照组相比Wnt2b蛋白的表达水平降低,miR-577抑制组与抑制对照组相比Wnt2b蛋白的表达水平升高(P<0.05)。miR-577+Wnt2b组与miR-577组相比,细胞活力升高,凋亡率降低(P<0.05)。结论:miR-577可通过下调Wnt/β-catenin信号通路Wnt2b蛋白表达抑制脑胶质瘤U251细胞活力,诱导细胞凋亡。
Abstract:
Aim:To investigate the effects of miR-577 on the viability and apoptosis of glioma cells U251 by regulating Wnt/β-catenin signaling pathway protein Wnt2b.Methods:U251 cells were divided into miR-control group,miR-577 group(cells were transfected with miR-577 mimics),inhibition control group,miR-577 inhibition group,and miR-577+Wnt2b group(simultaneously overexpressing miR-577 and Wnt2b). The expression of miR-577 was detected by qRT-PCR. Cell viability and apoptotic rate were detected by CCK-8 and flow cytometry, respectively. The targeting relationship between miR-577 and Wnt2b was validated by dual fluorescence reporter gene detection system.Results:Compared with the miR-control group, the expression level of miR-577 increased.The dual fluorescence reporter gene confirmed that Wnt2b was the target gene of miR-577, and the expression level of Wnt2b was significantly decreased in miR-577 group compared with miR-control group(P<0.05). Compared with inhibition control group,the expression level of Wnt2b in miR-577 inhibition group was significantly increased(P<0.05).Compared with miR-577 group, cell viability of miR-577+Wnt2b group increased,and apoptotic rate decreased(P<0.05).Conclusion:miR-577 could inhibit the viability of glioma cells U251 and induce apoptosis by down-regulating Wnt/β-catenin signaling pathway protein Wnt2b.

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备注/Memo

备注/Memo:
【基金项目】国家自然科学基金青年科学基金资助项目(81701357)
【作者简介】方兴根,通信作者,男,1969年3月生,博士,副主任医师,研究方向:脑血管病基础与临床,E-mail:Fangxinggen@163.com
更新日期/Last Update: 2019-07-20