[1]顾 然,王 露,唐 蔓,等.miR-422a过表达联合EPO对H2O2诱导的PC12细胞活力、凋亡及PI3K/AKT信号通路的影响[J].郑州大学学报(医学版),2019,(04):593-596.[doi:10.13705/j.issn.1671-6825.2018.11.079]
 GU Ran,WANG Lu,TANG Man,et al.Effects of miR-422a overexpression combined with EPO on viability, apoptosis and PI3K/AKT signaling pathway of PC12 cells induced by H2O2[J].JOURNAL OF ZHENGZHOU UNIVERSITY(MEDICAL SCIENCES),2019,(04):593-596.[doi:10.13705/j.issn.1671-6825.2018.11.079]
点击复制

miR-422a过表达联合EPO对H2O2诱导的PC12细胞活力、凋亡及PI3K/AKT信号通路的影响()
分享到:

《郑州大学学报(医学版)》[ISSN:1671-6825/CN:41-1340/R]

卷:
期数:
2019年04期
页码:
593-596
栏目:
应用研究
出版日期:
2019-07-20

文章信息/Info

Title:
Effects of miR-422a overexpression combined with EPO on viability, apoptosis and PI3K/AKT signaling pathway of PC12 cells induced by H2O2
作者:
顾 然王 露唐 蔓胡 晓
贵州省人民医院神经内科 贵阳 550002
Author(s):
GU Ran WANG LuTANG ManHU Xiao
Department of Neurology, Guizhou People's Hospital, Guiyang 550002
关键词:
PC12细胞 miR-422a 促红细胞生成素 凋亡 PI3K/AKT信号通路
Keywords:
PC12 cell miR-422a erythropoietin apoptosis PI3K/AKT signaling pathway
分类号:
R363
DOI:
10.13705/j.issn.1671-6825.2018.11.079
摘要:
目的:探讨过表达miR-422a和促红细胞生成素(EPO)单独或联合作用对过氧化氢(H2O2)诱导的PC12细胞活力、凋亡及PI3K/AKT信号通路的影响。方法:将PC12细胞分为空白组、H2O2组(300 μmol/L H2O2处理4 h)、miR-NC组(H2O2刺激前转染空质粒48 h)、miR-422a组(H2O2刺激前转染100 nmol/L miR-422a mimics 48 h)、EPO组(H2O2刺激前用1 IU/mL EPO处理细胞2 h); miR-422a+EPO组(H2O2刺激前同上,用EPO及miR-422a mimics处理细胞)。用qRT-PCR、MTT法、Annexin V-FITC/PI双染法及Western blot分别检测miR-422a mRNA表达、细胞活力、细胞凋亡及p-AKT蛋白表达。结果:H2O2刺激可降低PC12细胞miR-422a表达,转染miR-422a mimics后miR-422a的表达升高(P<0.05)。H2O2刺激可抑制细胞活力,促进细胞凋亡,下调p-AKT蛋白的表达; 过表达miR-422a及EPO均可增强细胞活力,抑制细胞凋亡,上调p-AKT表达,二者合用效应更强(P<0.05)。结论:过表达miR-422a及EPO均可增强H2O2诱导的PC12细胞活力,降低细胞凋亡率,两者合用效应更强,其机制可能与激活PI3K/AKT信号通路有关。
Abstract:
Aim:To investigate the effects of miR-422a overexpression or erythropoietin(EPO)alone or in combination on the viability, apoptosis and PI3K/AKT signaling pathway of PC12 cells induced by hydrogen peroxide(H2O2).Methods:PC12 cells were divided into blank group, H2O2 group(treated with 300 μmol/L H2O2 for 4 hours), miR-NC group(transfected empty plasmid before H2O2 stimulation), miR-422a group(transfected 100 nmol/L miR-422a mimics for 48 hours before H2O2 stimulation), EPO group(treated with 1 IU/mL EPO for 2 hours before H2O2 stimulation),and miR-422a+EPO group(treated with EPO and miR-422a mimics before H2O2 stimulation). The expression of miR-422a, cell viability, apoptosis and p-AKT protein expression were detected by qRT-PCR, MTT, Annexin V-FITC/PI double staining and Western blot,respectively.Results:The transfection of miR-422a mimics significantly increased the miR-422a expression of PC12 cells induced by H2O2. H2O2 stimulation could significantly inhibit cell viability, promote cell apoptosis, down-regulate p-AKT expression; while over-expression of miR-422a and EPO could both enhance cell viability, inhibit cell apoptosis and up-regulate p-AKT expression,and the combination of the two factors had more significant effects(P<0.05).Conclusion:Overexpression of miR-422a and EPO can enhance the viability and decrease the apoptotic rate of PC12 cells induced by H2O2. The combination of miR-422a overexpression and EPO has more significant effects. The mechanism may be related to the activation of PI3K/AKT signaling pathway.

参考文献/References:

[1] PAUL R,CHOUDHURY A,KUMAR S,et al.Cholesterol contributes to dopamine-neuronal loss in MPTP mouse model of Parkinson's disease: Involvement of mitochondrial dysfunctions and oxidative stress[J].PLoS One,2017,12(2):e0171285
[2] NAMIOKA N,HANYU H,HIROSE D,et al.Oxidative stress and inflammation are associated with physical frailty in patients with Alzheimer's disease[J].Geriatr Gerontol Int,2017,17(6):913
[3] 侯天芳, 王广发. MicroRNA在颗粒物诱导气道免疫炎症中的研究进展[J]. 国际呼吸杂志, 2017,37(24):1916
[4] 刘松.过氧化氢诱导PC12细胞凋亡中miR-422a作用机制初探[D].广州:中山大学,2008.
[5] 陈勤玲,蒋辉英,杨梅,等.促红细胞生成素对缺氧缺血性脑损伤保护作用机制的研究[J].中国儿童保健杂志,2016,24(8):825
[6] 张梅梅,闫方方,蒋超,等.重组人促红细胞生成素对短暂性缺氧新生大鼠的神经保护作用[J].郑州大学学报(医学版),2016,51(3):382
[7] SABERZADEH J,OMRANI M,TAKHSHID MA.Protective effects of nimodipine and lithium against aluminum-induced cell death and oxidative stress in PC12 cells[J].Iran J Basic Med Sci,2016,19(11):1251
[8] 刘倩,周庆.过氧化氢对人脐静脉内皮细胞白细胞介素-37表达的影响[J].蚌埠医学院学报,2016,41(11):1413
[9] YANG L,MU YP,CUI HW,et al.MiR-9-3p augments apoptosis induced by H2O2 through down regulation of Herpud1 in glioma[J].PLoS One,2017,12(4):e0174839
[10]SUN YQ,ZHU W,ZHOU SY,et al.Exploring the model of PC12 apoptosis induced by OGSD/R through in vitro experiments[J].Oncotarget,2017,8(52):90176
[11] 张艳茹. 白藜芦醇对H2O2诱导PC12细胞损伤的保护作用[D]. 唐山:河北联合大学, 2014.
[12]LIANG HQ,WANG RJ,JIN Y,et al.MiR-422a acts as a tumor suppressor in glioblastoma by targeting PIK3CA[J].Am J Cancer Res,2016,6(8):1695
[13]WU LA,HU B,ZHAO BT,et al.Circulating microRNA-422a is associated with lymphatic metastasis in lung cancer[J].Oncotarget,2017,8(26):42173
[14]WANG W,LI DS,LI Q,et al.Erythropoietin promotes peripheral nerve regeneration in rats by upregulating expression of insulin-like growth factor-1[J].Arch Med Sci,2015,11(2):433
[15]姚婕,赵蓓,姬文涛,等.RANTES对MPP+诱导的PC12细胞凋亡的保护作用及其机制研究[J].神经解剖学杂志,2016,32(5):647
[16]FU X,WEN HQ,JING L,et al.MicroRNA-155-5p promotes hepatocellular carcinoma progression by suppressing PTEN through the PI3K/Akt pathway[J].Cancer Sci,2017,108(4):620
[17]CHEN L,SUN L,LIU Z,et al.Protection afforded by quercetin against H2O2-induced apoptosis on PC12 cells via activating PI3K/Akt signal pathway[J].J Recept Signal Transduct Res,2015,36(1):1
[18]XU B,WANG GY,ZHANG JQ,et al.Resveratrol decreases FoXO protein expression through PI3K-Akt-dependent pathway inhibition in H2O2-treated synoviocytes[J].Histol Histopathol,2017,32(12):1305

相似文献/References:

[1]龚全峰)△,杨红旗).烷丙苯胺对6羟基多巴胺致PC12细胞损伤的改善作用[J].郑州大学学报(医学版),2009,(05):1003.
 GONG Quanfeng),YANG Hongqi).Neuroprotective effects of deprenyl against 6hydroxydopamine inducing neurotoxicity in PC12 cells[J].JOURNAL OF ZHENGZHOU UNIVERSITY(MEDICAL SCIENCES),2009,(04):1003.
[2]臧卫周△,杨红旗,徐军,等.姜黄素对缺氧复氧损伤PC12细胞的影响[J].郑州大学学报(医学版),2009,(03):594.
 ZANG Weizhou,YANG Hongqi,XU Jun,et al.Effects of curcumin on PC12 cells impaired by mimic ischemia and reperfusion[J].JOURNAL OF ZHENGZHOU UNIVERSITY(MEDICAL SCIENCES),2009,(04):594.
[3]潘帧婕,曹靖,李鸣,等.谷氨酸钠对PC12细胞钠离子通道蛋白1.7表达的影响*[J].郑州大学学报(医学版),2013,(05):591.
 PAN Zhenjie,CAO Jing,LI Ming,et al.Effects of sodium glutamate on sodium channel protein 1.7 subunit expression in PC12 cells[J].JOURNAL OF ZHENGZHOU UNIVERSITY(MEDICAL SCIENCES),2013,(04):591.
[4]任京力),林玲),王雁梅),等.氯通道阻断剂DIDS对过氧化氢诱导PC12细胞凋亡的影响*[J].郑州大学学报(医学版),2015,(01):17.
 REN Jingli),LIN Ling),WANG Yanmei),et al.Effect of chloride channel blocker DIDS on apoptosis of PC12 cells induced by hydrogen peroxide[J].JOURNAL OF ZHENGZHOU UNIVERSITY(MEDICAL SCIENCES),2015,(04):17.

备注/Memo

备注/Memo:
【基金项目】贵州省科技计划项目(黔科合LH字【2014】7022号)
【作者简介】胡晓,通信作者,女,1982年4月生,博士研究生,主任医师,研究方向:神经系统免疫疾病,E-mail:wopi51767465@163.com
更新日期/Last Update: 2019-07-20