[1]田文珺,刘士佳,刘晓明,等.异丙酚对卵巢癌SKOV3细胞克隆形成、侵袭、EMT和miR-143表达的影响[J].郑州大学学报(医学版),2019,(04):607-610.[doi:10.13705/j.issn.1671-6825.2019.02.003]
 TIAN Wenjun,LIU Shijia,LIU Xiaoming,et al.Effects of propofol on cloning formation,invasion,EMT and the expression of miR-143 in ovarian cancer SKOV3 cells[J].JOURNAL OF ZHENGZHOU UNIVERSITY(MEDICAL SCIENCES),2019,(04):607-610.[doi:10.13705/j.issn.1671-6825.2019.02.003]
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异丙酚对卵巢癌SKOV3细胞克隆形成、侵袭、EMT和miR-143表达的影响()
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《郑州大学学报(医学版)》[ISSN:1671-6825/CN:41-1340/R]

卷:
期数:
2019年04期
页码:
607-610
栏目:
应用研究
出版日期:
2019-07-20

文章信息/Info

Title:
Effects of propofol on cloning formation,invasion,EMT and the expression of miR-143 in ovarian cancer SKOV3 cells
作者:
田文珺刘士佳刘晓明吴 越
大连医科大学附属第一医院麻醉科 辽宁大连 116000
Author(s):
TIAN WenjunLIU ShijiaLIU XiaomingWU Yue
Department of Anesthesiology, the First Affiliated Hospital,Dalian Medical University,Dalian,Liaoning 116000
关键词:
异丙酚 miR-143 克隆形成 侵袭 上皮间质转化
Keywords:
propofol miR-143 cloning formation invasion epithelial mesenchymal transition
分类号:
R737.31
DOI:
10.13705/j.issn.1671-6825.2019.02.003
摘要:
目的:探讨异丙酚对卵巢癌SKOV3细胞克隆形成、侵袭、EMT和miR-143表达的影响。方法:以0.0、2.5、5.0和10.0 μmol/L异丙酚刺激SKOV3细胞48 h后,采用软琼脂集落形成实验检测细胞的克隆形成能力,Transwell小室实验检测细胞的侵袭能力,Western blot检测EMT相关蛋白E-cadherin和Vimentin的表达,实时荧光定量PCR检测细胞中miR-143的表达。脂质体法转染miR-143抑制剂后,观察下调miR-143表达对异丙酚刺激的SKOV3细胞的影响。结果:异丙酚能够呈浓度依赖性地抑制SKOV3细胞的克隆形成能力、侵袭能力和Vimentin蛋白的表达,促进E-cadherin蛋白和miR-143的表达(P<0.05)。转染miR-143抑制剂后,异丙酚对SKOV3细胞的上述作用明显逆转(P<0.05)。结论:异丙酚可能通过上调miR-143表达抑制SKOV3细胞克隆形成、侵袭和EMT进程,发挥抑制癌细胞转移的作用。
Abstract:
Aim:To investigate the effects of propofol on the cloning formation,invasive ability,epithelial mesenchymal transition,the expression of miR-143 of ovarian cancer SKOV3 cells and its mechanism.Methods:SKOV3 cells were stimulated with 0.0, 2.5,5.0 and 10.0 μmol/L propofol for 48 hours. The colony forming ability of SKOV3 cells was detected by soft agar colony formation assay, the invasive ability of SKOV3 cells was detected by Transwell chamber assay, the expressions of EMT-related proteins E-cadherin and Vimentin were detected by Western blot, and the expression of miR-143 in SKOV3 cells was detected by real-time fluorescence quantitative PCR. The effects of down-regulation of the expression of miR-143 on propofol-stimulated SKOV3 cells were observed after transfection of miR-143 inhibitors by liposome method.Results:The cloning forming ability, invasive ability and Vimentin protein expression of SKOV3 cells were inhibited and the expressions of E-cadherin protein and miR-143 were promoted by propofol in a concentration-dependent manner(P<0.05).The above effects of propofol on SKOV3 cells were reversed after transfection with the inhibitor of miR-143(P<0.05).Conclusion:Propofol can inhibit SKOV3 cell clone formation, invasion and EMT process by up-regulating the expression of miR-143.

参考文献/References:

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备注/Memo

备注/Memo:
【基金项目】辽宁省科学技术计划项目(2017225018)
【作者简介】吴越,通信作者,女,1979年9月生,硕士,副主任医师,研究方向:围术期器官保护,E-mail:longkuy716957@163.com
更新日期/Last Update: 2019-07-20