[1]莫 靓,韦 兵,贺大璞,等.RNU12 siRNA转染对EC9706细胞生物学特性的影响[J].郑州大学学报(医学版),2019,(05):672-675.[doi:10.13705/j.issn.1671-6825.2018.12.165]
 MO Liang,WEI Bing,HE Dapu,et al.Effects of RNU12 siRNA transfection on biological characteristics of EC9706 cells[J].JOURNAL OF ZHENGZHOU UNIVERSITY(MEDICAL SCIENCES),2019,(05):672-675.[doi:10.13705/j.issn.1671-6825.2018.12.165]
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RNU12 siRNA转染对EC9706细胞生物学特性的影响()
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《郑州大学学报(医学版)》[ISSN:1671-6825/CN:41-1340/R]

卷:
期数:
2019年05期
页码:
672-675
栏目:
食管癌研究
出版日期:
2019-09-20

文章信息/Info

Title:
Effects of RNU12 siRNA transfection on biological characteristics of EC9706 cells
作者:
莫 靓韦 兵贺大璞聂 军梁任技阳 志谢首智吴生荣
南华大学附属第一医院胸外科 湖南衡阳 421001
Author(s):
MO LiangWEI BingHE DapuNIE Jun LIANG RenjiYANG ZhiXIE ShouzhiWU Shengrong
Department of Thoracic Surgery, the First Hospital Affiliated to Nanhua University, Hengyang, Hunan 421001
关键词:
食管癌 LncRNA RNU12 EC9706细胞 增殖 凋亡 侵袭 迁移
Keywords:
esophageal cancer LncRNA RNU12 EC9706 cell proliferation apoptosis invasion migration
分类号:
R735.1
DOI:
10.13705/j.issn.1671-6825.2018.12.165
摘要:
目的:研究长链非编码RNA(LncRNA)RNU12 siRNA转染对食管癌EC9706细胞生物学特性的影响。方法:采用qRT-PCR检测EC9706细胞和人食管上皮细胞HEEC中RNU12的表达水平。将RNU12 siRNA转染EC9706细胞,同时设阴性对照组(转染随机序列)和空白对照组(未转染),采用qRT-PCR检测RNU12的表达,采用MTT法检测细胞增殖,Annexin V-FITC和PI双染法检测细胞凋亡,Transwell实验检测细胞侵袭和迁移能力。结果:EC9706细胞中RNU12的表达水平高于HEEC细胞(P<0.05)。与空白对照组和阴性对照组比较,RNU12 siRNA组细胞中RNU12的表达水平降低,细胞增殖能力受抑,凋亡率升高,侵袭和迁移细胞数减少(P<0.05)。结论:RNU12在EC9706细胞中高表达,干扰RNU12的表达能够抑制EC9706细胞增殖,促进细胞凋亡,阻碍细胞侵袭和迁移。
Abstract:
Aim:To investigate the effects of long non-coding RNA RNU12 on the biological characteristics of esophageal cancer EC9706 cells.Methods:The expression level of RNU12 in EC9706 cells and human esophageal epithelial cells HEEC was detected by qRT-PCR. RNU12 siRNA was transfected into EC9706 cells, and a negative control group(transfected random sequence)and a blank control group(not transfected)were set. The expression of RNU12 was detected by qRT-PCR.Cell proliferation was detected by MTT assay,apoptosis was detected by Annexin V-FITC and PI double staining,and Transwell assay was used to detect cell invasion and migration.Results:The expression level of RNU12 in EC9706 cells was higher than that in HEEC cells(P<0.05). Compared with the blank control group and the negative control group, the expression level of RNU12 was decreased in the RNU12 siRNA group, the cell proliferation ability was inhibited, the apoptosis rate was increased, and the number of invasion and migration cells was decreased(P<0.05).Conclusion:RNU12 is highly expressed in EC9706 cells. Interfering the expression of RNU12 could inhibit the proliferation of EC9706 cells, promote cell apoptosis, and hinder cell invasion and migration.

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备注/Memo

备注/Memo:
【基金项目】湖南省教育厅课题(17C1389); 2017年南华大学附属第一医院亚专科重点发展项目
【作者简介】莫靓,男,1976年5月生,硕士,副主任医师,副教授,研究方向:胸部肿瘤的综合治疗,E-mail:Moling208@163.com
更新日期/Last Update: 2019-09-20