[1]吴丹荣),李 红),王 超),等.生长分化因子15-siRNA对人垂体瘤HPAs细胞增殖、侵袭及NF-κB信号通路的影响[J].郑州大学学报(医学版),2019,(05):681-684.[doi:10.13705/j.issn.1671-6825.2018.10.170]
 WU Danrong),LI Hong),WANG Chao),et al.Effects of growth differentiation factor 15-siRNA on proliferation, invasion and NF-κB signaling pathway of human pituitary adenoma cells HPAs[J].JOURNAL OF ZHENGZHOU UNIVERSITY(MEDICAL SCIENCES),2019,(05):681-684.[doi:10.13705/j.issn.1671-6825.2018.10.170]
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生长分化因子15-siRNA对人垂体瘤HPAs细胞增殖、侵袭及NF-κB信号通路的影响()
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《郑州大学学报(医学版)》[ISSN:1671-6825/CN:41-1340/R]

卷:
期数:
2019年05期
页码:
681-684
栏目:
论著
出版日期:
2019-09-20

文章信息/Info

Title:
Effects of growth differentiation factor 15-siRNA on proliferation, invasion and NF-κB signaling pathway of human pituitary adenoma cells HPAs
作者:
吴丹荣1)李 红1)王 超2)高方友2)
1)贵州医科大学附属医院内分泌及代谢病科 贵阳 550004;2)贵州医科大学附属人民医院神经外科 贵阳 550002
Author(s):
WU Danrong1)LI Hong1)WANG Chao2)GAO Fangyou2)
1)Department of Endocrinology and Metabolism, the Affiliated Hospital, Guizhou Medical University, Guiyang 550004;2)Department of Neurosurgery, the Affiliated People's Hospital, Guizhou Medical University, Guiyang 550002
关键词:
垂体瘤 生长分化因子15 增殖 侵袭 NF-κB信号通路
Keywords:
pituitary tumor growth differentiation factor 15 proliferation invasion NF-κB signaling pathway
分类号:
R736.4
DOI:
10.13705/j.issn.1671-6825.2018.10.170
摘要:
目的:探讨生长分化因子15(GDF15)-siRNA对人垂体瘤HPAs细胞增殖、侵袭和NF-κB信号通路的影响。方法:将体外培养的人垂体瘤HPAs细胞分为对照组(未转染)、GDF15-siRNA组(转染GDF15-siRNA)和NC-siRNA组(转染阴性对照siRNA),采用Lipofectamine2000转染48 h后,qRT-PCR和Western blot检测转染效果,MTT法和Transwell小室分别检测细胞的增殖与侵袭能力,Western blot检测细胞中NF-κB p65磷酸化水平和MMP-9、VEGF蛋白的表达情况。结果:GDF15-siRNA组HPAs细胞的增殖和侵袭能力,细胞中GDF15、MMP-9、VEGF蛋白表达水平,NF-κB p65磷酸化水平以及GDF15 mRNA的表达水平较对照组均降低(P<0.05); 与对照组相比,NC-siRNA组细胞增殖、侵袭能力和NF-κB通路相关蛋白的表达无明显改变(P>0.05)。结论:GDF15-siRNA可抑制HPAs细胞的增殖和侵袭,其作用机制可能与抑制NF-κB信号通路的活化有关。
Abstract:
Aim:To investigate the effects of growth differentiation factor 15(GDF15)-siRNA on proliferation, invasion and NF-κB signaling pathway of human pituitary adenoma cells HPAs.Methods:HPAs cells cultured in vitro were divided into control group(untransfected), GDF15-siRNA group(transfected with GDF15-siRNA)and NC-siRNA group(transfected with negative control siRNA). After transfection with Lipofectamine2000 for 48 hours, the effects of transfection were detected by qRT-PCR and Western blot, the proliferation and invasion of cells in each group were checked by MTT and Transwell chambers, and the phosphorylation of NF-κB p65 and the expressions of MMP-9 and VEGF in each group were tested by Western blot.Results:After transfection with GDF15-siRNA, the proliferative and invasive ability of HPAs cells and the expression levels of GDF15, MMP-9, VEGF protein,the phosphorylation of NF-κB p65 and GDF15 mRNA were significantly lower than those of the control group(P<0.05). There was no significant difference in proliferation, invasion ability or expressions of NF-κB pathway-related proteins between NC-siRNA group and control group(P>0.05).Conclusion:GDF15-siRNA could inhibit the proliferation and invasion of HPAs cells, and its mechanism may be related to the inhibition of NF-κB signaling pathway activation.

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备注/Memo

备注/Memo:
【基金项目】贵州省科技厅-贵州医科大学联合基金项目(黔科合LG字[2012]040)
【作者简介】吴丹荣,女,1974年1月生,硕士,副教授,研究方向:糖尿病及其并发症、甲状腺疾病、骨代谢疾病,E-mail:wdr_174@163.com
更新日期/Last Update: 2019-09-20