[1]蔡治方,彭慈军,兑丹华,等.清胰Ⅱ号对牛黄胆酸钠诱导的急性胰腺炎大鼠的保护作用[J].郑州大学学报(医学版),2019,(05):754-757.[doi:10.13705/j.issn.1671-6825.2018.06.068]
 CAI Zhifang,PENG Cijun,DUI Danhua,et al.Protective effects of Qingyi Ⅱ on sodium taurocholate induced acute pancreatitis rats[J].JOURNAL OF ZHENGZHOU UNIVERSITY(MEDICAL SCIENCES),2019,(05):754-757.[doi:10.13705/j.issn.1671-6825.2018.06.068]
点击复制

清胰Ⅱ号对牛黄胆酸钠诱导的急性胰腺炎大鼠的保护作用()
分享到:

《郑州大学学报(医学版)》[ISSN:1671-6825/CN:41-1340/R]

卷:
期数:
2019年05期
页码:
754-757
栏目:
应用研究
出版日期:
2019-09-20

文章信息/Info

Title:
Protective effects of Qingyi Ⅱ on sodium taurocholate induced acute pancreatitis rats
作者:
蔡治方彭慈军兑丹华傲 宇兰天罡
遵义医学院附属医院肝胆外科 贵州遵义 563000
Author(s):
CAI ZhifangPENG CijunDUI DanhuaAO YuLAN Tiangang
Department of Hepatobiliary Surgery, the Affiliated Hospital, Zunyi Medical College, Zunyi, Guizhou 563000
关键词:
急性胰腺炎 大鼠 ROS JNK 清胰Ⅱ号
Keywords:
acute pancreatitis rat ROS JNK Qingyi Ⅱ
分类号:
R576
DOI:
10.13705/j.issn.1671-6825.2018.06.068
摘要:
目的:探讨清胰Ⅱ号对急性胰腺炎大鼠的保护作用及其可能的作用机制。方法:36只SD大鼠均分为正常组、模型组、治疗组。手术联合注射牛磺胆酸钠制备急性胰腺炎大鼠模型,术后正常组与模型组用生理盐水灌胃,治疗组用250 g/L清胰Ⅱ号按10 mL/kg灌胃,6 h一次。于第4次灌胃后处死大鼠收集胰腺、肠组织以及血液。胰腺组织HE染色后观察病理变化; 采用ELISA法检测大鼠血浆淀粉酶及血浆中IL-6、MCP-1含量; 采用双氢罗丹明-123法检测大鼠胰腺组织中ROS,氧化酶反应法检测大鼠胰腺组织中SOD,钼酸铵终止法检测CAT,硫代巴比妥酸法检测MDA; 细菌培养法检测大鼠肠道细菌数量以及分类; Western blot法检测大鼠胰腺组织中JNK蛋白的表达。结果:模型组与正常组比较,组织病理学评分、血浆淀粉酶活性、IL-6、MCP-1、ROS、MDA、肠道细菌数、JNK蛋白表达水平均升高,SOD、CAT降低(P<0.05); 治疗组与模型组比较,胰腺组织病理学评分、血浆淀粉酶活性、IL-6、MCP-1、ROS、MDA、肠道细菌数、JNK蛋白表达水平降低,SOD、CAT升高(P<0.05)。结论:清胰Ⅱ号对急性胰腺炎大鼠具有保护作用,其机制可能与抑制ROS/JNK通路有关。
Abstract:
Aim:To investigate the protective effect of Qingyi Ⅱ on acute pancreatitis rats and its related mechanism.Methods:Thirty-six SD rats were randomly allocated into normal group,model group and treatment group(12 in each group). Rat models of acute pancreatitis were prepared by injection of sodium taurocholate.Rats in the normal group and the model group were intragastrically administered with normal saline, and the treatment group was given 250 g/L Qingyi Ⅱ at 10 mL/kg every 6 hours.After the fourth intragastric administration, the rats were killed to collect pancreas, intestines and blood.The pathological changes of pancreatic tissue were observed after HE staining. ELISA was used to detect the plasma amylase activity and the contents of IL-6 and MCP-1 in plasma.ROS in the pancreas was detected by the method of double-hydrogen rhodamine-123.SOD in the pancreas of rats was detected by oxidase reaction.CAT and MDA were detected by ammonium molybdate colorimetry and thiobarbituric acid method. The number and classification of intestinal bacteria in rats were detected by bacterial culture. The JNK protein in the pancreatic tissue of rats was detected by Western blot.Results:Compared with normal group, histopathological score, plasma amylase activity, IL-6, MCP-1, ROS, MDA, intestinal bacteria count,and JNK protein expression level in the model group increased, while the levels of SOD and CAT decreased(P<0.05). Compared with the model group, the pathological score of pancreas, plasma amylase activity, IL-6, MCP-1, ROS, MDA, intestinal bacterial count and JNK protein expression level in the treatment group decreased,while the levels of SOD and CAT increased(P<0.05).Conclusion:Qingyi Ⅱ has obvious protective effects on acute pancreatitis model rats, and its mechanism may be related to inhibition of ROS/JNK pathway.

参考文献/References:

[1] LANKISCH PG,APTE M,BANKS PA.Acute pancreatitis[J].Lancet,2015,386(9988):85
[2] 项红,王治洲,尚东.清胰汤治疗急性胰腺炎的作用及分子药理机制[J].时珍国医国药,2018,29(2):408
[3] 朱风尚,朱国英,黄东平,等.基因芯片技术研究清胰汤对重症急性胰腺炎大鼠基因表达谱的影响[J].中国中西医结合杂志,2014,34(1):51
[4] 赵萍,张梦,石晓丹,等.胰腺良性及低度恶性肿瘤临床病理及诊治分析[J].郑州大学学报(医学版),2017,52(2):226
[5] 杨珠莹,谢齐贵,陈湛蕾,等.血清CRP与PCT及脂肪酶对重症急性胰腺炎患者发生感染性胰腺坏死的预测[J].中华医院感染学杂志,2018,28(11):1693
[6] 赵芳超.清热通腑活血汤内服外用对重症急性胰腺炎患者血清(胰)淀粉酶的影响[J].辽宁中医杂志,2018,45(5):1002
[7] 王齐,卢成康,李琴,等.乌司他丁联合连续静-静脉血液透析滤过治疗重症急性胰腺炎的疗效分析[J].河北医学,2017,23(4):564
[8] 张敏剑,李军成,吴伟兵,等.JNK/SAPK和NF-κB/IκB信号通路在重症急性胰腺炎肺损伤中的作用[J].实用医学杂志,2010,26(1):24
[9] 刘明东.JNK通路在急性胰腺炎相关性肺损伤的作用机制及丹参酮ⅡA的干预作用研究[D].南京:南京中医药大学,2010.
[10]李彩霞,张勇刚.早期鼻空肠营养给予整蛋白制剂与短肽制剂治疗急性重症胰腺炎的对比分析[J].河北医学,2018,24(4):548
[11]刘思义.高脂血症性胰腺炎与急性胆源性胰腺炎的特征比较[J].蚌埠医学院学报,2016,41(8):1045
[12]陈辰,石乔,余佳,等.氢饱和生理盐水对重症急性胰腺炎肺损伤的作用及对NOX2/ROS/NF-κB通路的影响[J].中国普通外科杂志,2016,25(3):363
[13]李敏,林思思,沈利,等.清胰汤Ⅱ号冲剂对急性胰腺炎小鼠的保护作用及机制[J].中国病理生理杂志,2015,31(7):1282
[14]阙日升,丁国平,刘达人,等.急性胰腺炎红细胞氧化应激与血液流变学的变化及意义[J].中华急诊医学杂志,2013,22(4):420
[15]THONGSOM S,SUGINTA W,LEE KJ,et al.Piperlongumine induces G2/M phase arrest and apoptosis in cholangiocarcinoma cells through the ROS-JNK-ERK signaling pathway[J].Apoptosis,2017,22(11):1473

相似文献/References:

[1]李红),宋辉).急性胆源性胰腺炎51例磁共振胆道造影和MRI诊断[J].郑州大学学报(医学版),2009,(04):918.
[2]俞悦),王震),穆永慧),等.自发性高血压大鼠学习记忆能力和脑组织SK2蛋白的表达*[J].郑州大学学报(医学版),2013,(06):746.
 YU Yue),WANG Zhen),MU Yonghui),et al.Expression of SK protein in brain tissue and the learning and memory capacity of spontaneously hypertensive rat[J].JOURNAL OF ZHENGZHOU UNIVERSITY(MEDICAL SCIENCES),2013,(05):746.
[3]方立),王雪晶),荆婧),等.rhG-CSF对大鼠缺血缺氧神经元巨自噬水平的影响*[J].郑州大学学报(医学版),2013,(06):753.
 FANG Li),WANG Xuejing),JING Jing),et al.Effects of rhG-CSF on macroautophagy of ischemia hypoxia SH-SY5Y cells and rat neurons[J].JOURNAL OF ZHENGZHOU UNIVERSITY(MEDICAL SCIENCES),2013,(05):753.
[4]韩俊),王彬),马珂),等.褪黑素对睡眠剥夺大鼠胃黏膜损伤的保护作用*[J].郑州大学学报(医学版),2013,(06):769.
 HAN Jun),WANG Bin),MA Ke),et al.Protective effects of melatonin on gastric mucosal lesions in rats induced by sleep deprivation[J].JOURNAL OF ZHENGZHOU UNIVERSITY(MEDICAL SCIENCES),2013,(05):769.
[5]杨晓村),王 颖)#,燕树勋),等.肾血管性高血压大鼠肾组织睾酮受体蛋白及丝裂素活化蛋白激酶磷酸酶1的表达[J].郑州大学学报(医学版),2015,(05):614.
 YANG Xiaocun)),WANG Ying)),YAN Shuxun),et al.Expressions of androgen receptor and mitogen-activated protein kinase phosphatase-1 in rats with renovascular hypertension[J].JOURNAL OF ZHENGZHOU UNIVERSITY(MEDICAL SCIENCES),2015,(05):614.
[6]刘海燕,娄季宇#,白宏英,等.脑梗死大鼠缺血脑组织中Slit2蛋白的表达及与血管新生的关系[J].郑州大学学报(医学版),2015,(05):655.
 LIU Haiyan,LOU Jiyu,BAI Hongying,et al.Expression of Slit2 protein in infarction brain tissue and its correlation with angiogenesis in rats with cerebral infarction[J].JOURNAL OF ZHENGZHOU UNIVERSITY(MEDICAL SCIENCES),2015,(05):655.
[7]李学银),张卫星),张天标),等.两种方式注射白消安对SD大鼠生精功能的影响[J].郑州大学学报(医学版),2015,(05):665.
 LI Xueyin),ZHANG Weixing),ZHANG Tianbiao),et al.Influence of two methods injecting busulfan to SD rats on spermatogenic function[J].JOURNAL OF ZHENGZHOU UNIVERSITY(MEDICAL SCIENCES),2015,(05):665.
[8]谢 毅△,张 超.精氨酸联合加味大柴胡汤对急性胆管炎大鼠肝脏功能的保护作用[J].郑州大学学报(医学版),2015,(05):706.
 XIE Yi,ZHANG Chao.Protective effects of arginine and modified major decoction of bupleurum on liver of rats with acute cholangitis[J].JOURNAL OF ZHENGZHOU UNIVERSITY(MEDICAL SCIENCES),2015,(05):706.
[9]常 悦),王明洁),王 月),等.不同正畸力对大鼠牙周组织张力侧BMP-2蛋白表达的影响[J].郑州大学学报(医学版),2015,(05):675.
 CHANG Yue),WANG Mingjie),WANG Yue),et al.Effects of different orthodontic force on expression of BMP-2 in periodontal tissue of tension side[J].JOURNAL OF ZHENGZHOU UNIVERSITY(MEDICAL SCIENCES),2015,(05):675.
[10]闫淑芳,吴 敬,刘宏霞,等.PDX-1联合利拉鲁肽诱导大鼠骨髓间充质干细胞分化为胰岛样细胞的效果*[J].郑州大学学报(医学版),2016,(02):161.
 YAN Shufang,WU Jing,LIU Hongxia,et al.Effect of PDX-1 combined with liraglutide on differentiation of bone marrow mesenchymal stem cells into insulin-producing cells[J].JOURNAL OF ZHENGZHOU UNIVERSITY(MEDICAL SCIENCES),2016,(05):161.

备注/Memo

备注/Memo:
【基金项目】贵州省科技计划项目(黔科合LH字[2016]7478)
【作者简介】蔡治方,男,1978年1月生,硕士,副教授,研究方向:胰腺炎的基础与临床,E-mail:nbz533111@163.com
更新日期/Last Update: 2019-09-20