[1]周馨魁),马珊珊),黄团结),等.MG53蛋白联合hUC-MSCs移植对阿尔兹海默病小鼠行为和情绪失调的改善作用[J].郑州大学学报(医学版),2019,(06):793-797.[doi:10.13705/j.issn.1671-6825.2018.10.098]
 ZHOU Xinkui),MA Shanshan),HUANG Tuanjie),et al.Improvement of behavioral and emotional dysregulation of Alzheimer's disease mice treated with MG53 protein and hUC-MSCs transplantation[J].JOURNAL OF ZHENGZHOU UNIVERSITY(MEDICAL SCIENCES),2019,(06):793-797.[doi:10.13705/j.issn.1671-6825.2018.10.098]
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MG53蛋白联合hUC-MSCs移植对阿尔兹海默病小鼠行为和情绪失调的改善作用()
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《郑州大学学报(医学版)》[ISSN:1671-6825/CN:41-1340/R]

卷:
期数:
2019年06期
页码:
793-797
栏目:
论著
出版日期:
2019-11-20

文章信息/Info

Title:
Improvement of behavioral and emotional dysregulation of Alzheimer's disease mice treated with MG53 protein and hUC-MSCs transplantation
作者:
周馨魁1)马珊珊1)黄团结1)李 喆1)崔渊博1)王亚苹1)刘腾飞1)周建康1)杨 波2)关方霞12)
1)郑州大学生命科学学院 郑州 450001 2)郑州大学第一附属医院神经外科 郑州 450052
Author(s):
ZHOU Xinkui1)MA Shanshan1)HUANG Tuanjie1)LI Zhe1)CUI Yuanbo1)WANG Yaping1)LIU Tengfei1)ZHOU Jiankang1)YANG Bo2)GUAN Fangxia12)
1)School of Life Sciences,Zhengzhou University,Zhengzhou 450001 2)Department of Neurosurgery, the First Affiliated Hospital,Zhengzhou University,Zhengzhou 450052
关键词:
MG53蛋白 人脐带间充质干细胞 Nrf2通路 阿尔兹海默病 小鼠
Keywords:
MG53 protein hUC-MSCs Nrf2 pathway Alzheimer's disease mouse
分类号:
R749
DOI:
10.13705/j.issn.1671-6825.2018.10.098
摘要:
目的:研究MG53蛋白联合人脐带间充质干细胞(hUC-MSCs)移植对阿尔茨海默病(AD)小鼠行为和情绪失调的改善作用。方法:将3月龄AD小鼠随机分为APP+组、MG53组、hUC-MSCs组和联合组,每组8只。APP+组小鼠尾静脉注射0.1 mL 生理盐水, hUC-MSCs组同法注射0.1 mL细胞密度为1×107 个/mL的hUC-MSCs悬液, MG53组按3 mg/kg注射0.1 mL rhMG53,联合组小鼠尾静脉注射含hUC-MSCs和rhMG53的混合液,1次/d,连续3 d。治疗3 d后,DHE染色法检测小鼠(n=3)脑内ROS含量; 治疗后第26~28天,采用旷场实验、新事物识别实验和新奇抑制喂养实验(n=4)评价小鼠的记忆、认知能力和抑郁情绪; 治疗后第29天,进行脑组织尼氏染色(n=4),硫磺素S染色法检测Aβ斑块面积(n=4),Western blot法检测Nrf2通路相关蛋白Nrf2、NQO1和Keap-1的表达(n=1)。结果:MG53和hUC-MSCs移植单独应用均可增加AD小鼠脑内尼氏小体数量,降低ROS含量,减小Aβ斑块面积,改善小鼠的学习记忆能力和焦虑情绪(P均<0.05)。二者联用有协同增效的作用(P均<0.05)。MG53和hUC-MSCs移植可以促进AD小鼠脑组织中Nrf2、NQO1蛋白的表达,降低Keap-1蛋白的表达。结论:MG53蛋白联合hUC-MSCs 移植可以激活Nrf2通路,在一定程度上改善AD小鼠的行为能力。
Abstract:
Aim:To study the effects of MG53 protein combined with hUC-MSCs transplantation on the behavior and emotion of Alzheimer's disease(AD)mice.Methods:Three-month-old AD mice were randomly allocated into APP+ group, MG53 group, hUC-MSCs group and hUC-MSCs+MG53 group.The mice were injected with 0.1 mL normal saline via tail vein in the APP+ group, with 0.1 mL hUC-MSC suspension of 1 × 107 cells/mL in the hUC-MSCs group, with 0.1 mL rhMG53 at dose of 3 mg/kg in the MG53 group, and with a mixture of hUC-MSCs and rhMG53 in hUC-MSCs+MG53 group, once a day for 3 days.After 3 days,the content of ROS in the cells was detected by DHE staining(n=3); on the 26th to 28th day, the memory ability,cognitive ability,and depressive mood of mice were evaluated by open field experiment,new object recognition experiment,and novelty-suppressed feeding experiment(n=4); on the 29th day,the development of neuronal corpuscles was detected by Nissl staining(n=4), the deposition of Aβ plaque was detected by thioflavin S staining(n=4),and the expressions of Nrf2 pathway related proteins(Nrf2,NQO1 and Keap-1)were detected by Western blot(n=1).Results:Both MG53 protein and hUC-MSCs transplantation alone could increase the number of neurons in the brain of AD mice, decrease the ROS content and Aβ deposition, improve the cognitive function and anxiety of the mice(P<0.05),and the combination of the two had synergistic effects(P<0.05).In addition, MG53 and hUC-MSCs promoted the expressions of Nrf2,NQO1 and decreased the expression of Keap-1 protein in the brain tissue of AD mice.Conclusion:MG53 protein combined with hUC-MSCs can activate Nrf2 pathway and improve the behavior of AD mice to some extent.

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备注/Memo

备注/Memo:
【基金项目】国家自然科学基金项目(81601078,81471306); 河南省重点科技攻关项目(152102310272); 河南省高等学校重点科研项目计划 (17A310012) 【作者简介】关方霞,通信作者,女,1969年2月生,博士,教授,研究方向:干细胞与再生医学,E-mail:guanfangxia@126.com; 马珊珊,通信作者,女,1984年5月生,博士,副教授,研究方向:神经修复相关的基础研究,E-mail:mashanshan84@163.com
更新日期/Last Update: 2019-11-20