[1]刘嘉研),李俊峰),车 楠),等.梓醇对哮喘小鼠气道炎症及AMPK/ROS/NF-κB信号通路的影响[J].郑州大学学报(医学版),2019,(06):823-827.[doi:10.13705/j.issn.1671-6825.2018.11.092]
　LIU Jiayan),LI Junfeng),CHE Nan),et al.Catalpol suppresses airway inflammation via AMPK/ROS/NF-κB signaling pathway in asthmatic mice[J].JOURNAL OF ZHENGZHOU UNIVERSITY(MEDICAL SCIENCES),2019,(06):823-827.[doi:10.13705/j.issn.1671-6825.2018.11.092]
点击复制

梓醇对哮喘小鼠气道炎症及AMPK/ROS/NF-κB信号通路的影响()
分享到：

《郑州大学学报(医学版)》[ISSN:1671-6825/CN:41-1340/R]

卷:
期数:
2019年06期
页码:
823-827
栏目:
论著
出版日期:
2019-11-20

文章信息/Info

Title:
Catalpol suppresses airway inflammation via AMPK/ROS/NF-κB signaling pathway in asthmatic mice
作者:
刘嘉研1); 李俊峰1; 2); 车 楠1; 2); 李 莉1; 2); 姜京植1; 2); 李良昌1; 2)
1)延边大学医学院 吉林延吉 133002 2)吉林省科技厅过敏性疾病重点实验室 吉林延吉 133002
Author(s):
LIU Jiayan1);  LI Junfeng1; 2);  CHE Nan1; 2);  LI Li1; 2);  JIANG Jingzhi1; 2);  LI Liangchang1; 2)
1)Medical College, Yanbian University,Yanji, Jilin 1330022)Key Laboratory of Allergic Diseases, Jilin Provincial Science and Technology Agency, Yanji, Jilin 133002
关键词:
梓醇;  哮喘;  氧化应激;  活性氧;  腺苷酸活化蛋白激酶;  小鼠
Keywords:
catalpol;  asthma;  oxidative stress;  reactive oxygen;  AMP-activated protein kinase;  mouse
分类号:
R322.3
DOI:
10.13705/j.issn.1671-6825.2018.11.092
摘要:
目的:从氧化应激角度探讨梓醇对哮喘气道炎症的影响及可能的作用机制。方法:40只雌性BALB/c小鼠随机分为正常对照组、模型组、梓醇低剂量组、梓醇高剂量组,每组10只; 后3组小鼠利用卵清蛋白诱导建立哮喘小鼠模型,梓醇低剂量组和高剂量组小鼠在卵清蛋白致敏第21天、激发前1 h灌服梓醇50或100 mg/(kg·d),共计7 d。检测各组小鼠气道高反应性,采用HE染色观察肺组织病理学改变,Diff-quik染色后计数支气管肺泡灌洗液(BALF)中各类炎症细胞,采用ELISA法检测BALF中白介素(IL)-4、IL-5和IL-13的含量,分别用流式细胞术和比色法检测小鼠肺组织中ROS水平、SOD活性和MDA含量,用Western blot法检测肺组织中NF-κB(p65)蛋白的表达以及腺苷酸活化蛋白激酶(AMPK)的磷酸化水平。结果:与模型组比较,梓醇尤其是高剂量梓醇可改善哮喘小鼠气道高反应性及肺组织病理改变; 降低哮喘小鼠BALF中炎症细胞数量以及IL-4、IL-5和IL-13的水平; 降低肺组织中ROS水平和MDA含量,提高SOD活性; 提高AMPK磷酸化水平,抑制NF-κB(p65)的表达(P<0.05)。结论:梓醇能够改善哮喘小鼠肺部氧化应激状态,并通过AMPK/ROS/NF-κB信号通路调控哮喘气道炎症。
Abstract:
Aim:To investigate the effects and possible mechanism of catalpol on asthma airway inflammation from the perspective of oxidative stress.Methods: Forty female BALB/c mice were randomly allocated into normal control group, model group, low-dose and high-dose catalpol groups[given 50 and 100 mg/(kg·d)catalpol]. The rats in the latter 3 groups were induced asthmatic model by ovalbumin. Airway hyperreactivity was measured. The lung tissue inflammation was observed by HE staining. The numbers of inflammatory cells in bronchoalveolar lavage fluid(BALF)were observed after Diff-quik staining. ELISA was used to detect the concentrations of interleukin-4(IL-4), IL-5, and IL-13 in BALF. The level of ROS in the lung tissue was detected by flow cytometry, and the activity of SOD and the content of MDA were detected by colorimetry. Western blot was used to detect the expression levels of NF-κB(p65)and phosphorylation of AMPK in lung tissue.Results: Compared with model group, the numbers of inflammatory cells and the expression levels of IL-4, IL-5, and IL-13 in BALF in low-dose and high-dose catalpol groups reduced,the pathological changes in lung tissue and airway hyperresponsiveness improved, the contents of ROS and MDA decreased, and the activity of SOD in lung tissue enhanced,the phosphorylation of AMPK increased and the expression of NF-κB(p65)was inhibited(P<0.05),especially in high-dose catalpol group.Conclusion: Catalpol can restrain oxidative stress and suppress airway inflammation via AMPK/ROS/NF-κB signaling pathway in asthmatic mice.

参考文献/References:

[1] 何方婷, 陈嘉熠, 徐佳伊, 等. 氧化应激细胞模型建立的研究进展[J]. 食品工业科技,2019,40(7):341
[2] ANTUNES GL, SILVEIRA JS,KAIBER DB,et al.Cholinergic anti-inflammatory pathway confers airway protection against oxidative damage and attenuates inflammation in an allergic asthma model[J].J Cell Physiol,2019. doi: 10.1002/jcp.29101
[3] ZHANG XL,JIANG B,LI ZB,et al.Catalpol ameliorates cognition deficits and attenuates oxidative damage in the brain of senescent mice induced by D-galactose[J].Pharmacol Biochem Behav,2007,88(1):64
[4] CHEN YY,ZHANG YZ,XU MY,et al.Catalpol alleviates ovalbumin-induced asthma in mice:reduced eosinophil infiltration in the lung[J].Int Immunopharmacol,2017,43:140
[5] LI LC,PIAO HM,ZHENG MY,et al.Ginsenoside Rh2 attenuates allergic airway inflammation by modulating nuclear factor-κB activation in a murine model of asthma[J].Mol Med Rep,2015,12(5):6946
[6] LI J,ZHONG LP,WANG FZ,et al.Dissecting the role of AMP-activated protein kinase in human diseases[J].Acta Pharm Sin B,2017,7(3):249
[7] FAUBERT B,VINCENT EE,GRISS T,et al.Loss of the tumor suppressor LKB1 promotes metabolic reprogramming of cancer cells via HIF-1α[J].Proc Natl Acad Sci U S A,2014,111(7):2554
[8] PHULL AR,NASIR B,HAQ IU,et al.Oxidative stress, consequences and ROS mediated cellular signaling in rheumatoid arthritis[J].Chem Biol Interact,2018,281:121

相似文献/References:

[1]杜芳宇,刘剑波#.吡格列酮对哮喘小鼠肺组织GABAARα、MUC5AC表达的影响*[J].郑州大学学报(医学版),2014,(01):33.
　DU Fangyu,LIU Jianbo.Influence of pioglitazone on expressions of GABAARα and MUC5AC in lung tissue of asthmatic mice[J].JOURNAL OF ZHENGZHOU UNIVERSITY(MEDICAL SCIENCES),2014,(06):33.

备注/Memo

备注/Memo:
【基金项目】国家自然科学基金项目(81560004); 吉林省大学生创新项目(201810184104) 【作者简介】李良昌,通信作者,男,1978年5月生,博士,副教授,研究方向:过敏性疾病,E-mail:lclee@ybu.edu.cn
更新日期/Last Update: 2019-11-20