[1]马 媛),张大鹏),王 想),等.lncRNA TUG1对高糖诱导的小鼠足细胞MPC5凋亡的影响[J].郑州大学学报(医学版),2019,(06):863-866.[doi:10.13705/j.issn.1671-6825.2019.06.072]
 MA Yuan),ZHANG Dapeng),WANG Xiang),et al.Effects of lncRNA TUG1 on high glucose-induced apoptosis of mouse podocyte MPC5[J].JOURNAL OF ZHENGZHOU UNIVERSITY(MEDICAL SCIENCES),2019,(06):863-866.[doi:10.13705/j.issn.1671-6825.2019.06.072]
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lncRNA TUG1对高糖诱导的小鼠足细胞MPC5凋亡的影响()
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《郑州大学学报(医学版)》[ISSN:1671-6825/CN:41-1340/R]

卷:
期数:
2019年06期
页码:
863-866
栏目:
应用研究
出版日期:
2019-11-20

文章信息/Info

Title:
Effects of lncRNA TUG1 on high glucose-induced apoptosis of mouse podocyte MPC5
作者:
马 媛1)张大鹏1)王 想1)任 蕾2)
1)驻马店市中心医院内分泌科 河南驻马店 463000 2)郑州大学第一附属医院内分泌科 郑州 450052
Author(s):
MA Yuan1)ZHANG Dapeng1)WANG Xiang1)REN Lei2)
1)Department of Endocrinology, Zhumadian Central Hospital, Zhumadian, Henan 463000 2)Department of Endocrinology, the First Affiliated Hospital, Zhengzhou University, Zhengzhou 450052
关键词:
lncRNA TUG1 足细胞 糖尿病 凋亡 MPC5细胞 小鼠
Keywords:
lncRNA TUG1 podocyte diabetes apoptosis MPC5 cell mouse
分类号:
R587.2
DOI:
10.13705/j.issn.1671-6825.2019.06.072
摘要:
目的:探讨长链非编码RNA牛磺酸调节基因1(lncRNA TUG1)对高糖诱导的足细胞MPC5凋亡的影响。方法:小鼠足细胞MPC5分成对照组、高糖组、阴性对照高糖组、TUG1过表达高糖组,培养48 h后采用qRT-PCR检测TUG1表达变化,PI及Annexin V-FITC染色后采用流式细胞术测定细胞凋亡率,Western blot法检测细胞中Cleaved-Caspase-12、Cleaved-PARP、CHOP、p-eIF2α、GRP78蛋白表达水平。结果:与对照组比较,高糖组细胞中TUG1表达水平降低,细胞凋亡率和Cleaved-Caspase-12、Cleaved-PARP、CHOP、p-eIF2α、GRP78蛋白表达水平增加(P<0.05)。与阴性对照高糖组比较,TUG1过表达高糖组细胞中TUG1表达水平升高,细胞凋亡率和Cleaved-Caspase-12、Cleaved-PARP、CHOP、p-eIF2α、GRP78蛋白表达水平下降(P<0.05)。结论:lncRNA TUG1可抑制高糖诱导的足细胞MPC5凋亡,作用机制可能与降低内质网应激有关。
Abstract:
Aim:To investigate the effects of long non-coding RNA taurine up-regulated gene 1(lncRNA TUG1)on apoptosis of podocytes induced by high glucose in mouse podocyte MPC5.Methods:Mouse podocyte MPC5 was divided into control group, high glucose group, high glucose culture group(high glucose+lentivirus infection with negative control),TUG1+high glucose group(high glucose+TUG1 overexpression lentivirus infection). After being cultured for 48 hours, qRT-PCR was used to detect the expression of TUG1; flow cytometry was used to detect cell apoptosis after PI and Annexin V-FITC staining; Western blot was used to detect the expressions of Cleaved-Caspase-12, Cleaved-PARP, CHOP, p-eIF2α and GRP78 proteins in cells.Results:Compared with control group, the expression of TUG1 was decreased in the high glucose group, and the apoptosis rate and expressions of Cleaved-Caspase-12, Cleaved-PARP, CHOP, p-eIF2α and GRP78 proteins were increased(P<0.05). Compared with high glucose culture group,the expression of TUG1 was increased in the TUG1+high glucose group,the apoptosis rate and expressions of Cleaved-Caspase-12, Cleaved-PARP,CHOP,p-eIF2α and GRP78 proteins were decreased(P<0.05).Conclusion:lncRNA TUG1 could inhibit apoptosis of podocytes MPC5 induced by high glucose, and the mechanism may be related to the reduction of endoplasmic reticulum stress.

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备注/Memo

备注/Memo:
【基金项目】河南省医学科技攻关计划项目(201802015) 【作者简介】马媛,女,1984年9月生,硕士,主治医师,研究方向:糖尿病,E-mail:mayuan19840902@163.com
更新日期/Last Update: 2019-11-20