[1]葛路路),石 雁),朱静静),等.沉默FOXC1表达对A549细胞化疗敏感性的影响[J].郑州大学学报(医学版),2019,(06):870-874.[doi:10.13705/j.issn.1671-6825.2018.12.064]
 GE Lulu),SHI Yan),ZHU Jingjing),et al.Effects of silencing FOXC1 on chemotherapy sensitivity of A549 cells[J].JOURNAL OF ZHENGZHOU UNIVERSITY(MEDICAL SCIENCES),2019,(06):870-874.[doi:10.13705/j.issn.1671-6825.2018.12.064]
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沉默FOXC1表达对A549细胞化疗敏感性的影响()
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《郑州大学学报(医学版)》[ISSN:1671-6825/CN:41-1340/R]

卷:
期数:
2019年06期
页码:
870-874
栏目:
应用研究
出版日期:
2019-11-20

文章信息/Info

Title:
Effects of silencing FOXC1 on chemotherapy sensitivity of A549 cells
作者:
葛路路1)石 雁2)朱静静1)李艳娟1)马东波1)王 静1)吴秋歌1)
1)郑州大学第一附属医院呼吸与危重症医学科 郑州 450052 2)新乡市中心医院呼吸内科 河南新乡 453000
Author(s):
GE Lulu1) SHI Yan2) ZHU Jingjing1) LI Yanjuan1) MA Dongbo1) WANG Jing1) WU Qiuge1)
1)Department of Respiratory and Critical Care Medicine,the First Affiliated Hospital,Zhengzhou University,Zhengzhou,450052 2)Department of Respiratory Medicine, Xinxiang Central Hospital, Xinxiang,Henan 453000
关键词:
FOXC1 A549细胞 顺铂 化疗敏感性
Keywords:
FOXC1 A549 cell cisplatin chemotherapy sensitivity
分类号:
R734.2
DOI:
10.13705/j.issn.1671-6825.2018.12.064
摘要:
目的:探讨沉默A549中的转录因子叉头框C1(FOXC1)表达对其顺铂化疗敏感性的影响及可能机制。方法:取对数生长期A549细胞,分别转染NC siRNA和FOXC1 siRNA后加入顺铂处理细胞。根据细胞是否转染FOXC1 siRNA和顺铂处理将A549细胞分为空白对照组、转染试剂组、转染NC siRNA组、转染FOXC1 siRNA组、转染NC siRNA+顺铂组和转染FOXC1 siRNA+顺铂组。采用CCK-8法检测细胞增殖抑制情况; 双染法检测细胞凋亡; qRT-PCR和Western blot检测各组细胞中FOXC1、抗凋亡相关因子Bcl-2和促凋亡相关因子Bax mRNA及蛋白的表达情况。结果:与其他各组相比,FOXC1 siRNA+顺铂组A549细胞增殖受抑制(P<0.05),凋亡率明显增高(P<0.05); FOXC1和Bcl-2蛋白及其mRNA表达下降(P<0.05),而Bax蛋白及其mRNA表达升高(P<0.05)。结论:抑制FOXC1表达可能通过调控内源性凋亡通路来提高A549细胞对顺铂的化疗敏感性。
Abstract:
Aim:To observe the impact of silencing forkhead box transcription factor C1(FOXC1)gene on the chemosensitivity of non-small carcinoma cells A549 to cisplatin.Methods:A549 cells at logarithmic growth phase were treated with cisplatin after being transfected with FOXC1 siRNA and NC siRNA, respectively. Based on the different treatments, the A549 cells were divided into six groups:blank group, transfection reagent group, NC siRNA group, FOXC1 siRNA group, NC siRNA+ cisplatin and FOXC1 siRNA+cisplatin group. The cell proliferation and cell apoptosis were detected through CCK-8 assay and double staing, respectively. The expressions of FOXC1, Bcl-2 and Bax mRNA and protein were analyzed using qRT-PCR and Western blot, respectively.Results:Compared with other groups, the proliferation of A549 cells in FOXC1 siRNA+cisplatin group while the apoptosis rate was increased(P<0.05).the expressions of FOXC1 and Bcl-2 mRNA and protein were lower(P<0.05), while that of Bax mRNA and protein was higher(P<0.05).Conclusion:The inhibition of FOXC1 could improve the sensitivity of A549 cells to cisplatin by regulating the endogenous apoptotic pathway.

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备注/Memo

备注/Memo:
【基金项目】河南省医学科技攻关计划普通项目(201602045) 【作者简介】吴秋歌,通信作者,女,1975年3月生,博士,副教授,研究方向:肺癌的发病机制和早期预警,E-mail:wqgjh@126.com
更新日期/Last Update: 2019-11-20