[1]张雪燕),李蕾蕾),石晓丽),等.木犀草素对喉癌Hep2细胞凋亡的影响[J].郑州大学学报(医学版),2019,(06):883-886.[doi:10.13705/j.issn.1671-6825.2018.12.027]
 ZHANG Xueyan),LI Leilei),SHI Xiaoli),et al.Effects of luteolin on apoptosis of laryngeal cancer Hep2 cells[J].JOURNAL OF ZHENGZHOU UNIVERSITY(MEDICAL SCIENCES),2019,(06):883-886.[doi:10.13705/j.issn.1671-6825.2018.12.027]
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木犀草素对喉癌Hep2细胞凋亡的影响()
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《郑州大学学报(医学版)》[ISSN:1671-6825/CN:41-1340/R]

卷:
期数:
2019年06期
页码:
883-886
栏目:
应用研究
出版日期:
2019-11-20

文章信息/Info

Title:
Effects of luteolin on apoptosis of laryngeal cancer Hep2 cells
作者:
张雪燕1)李蕾蕾1)石晓丽2)霍峻锋1)刘晓洁1)秦甜甜1)刘卫华1)申益维1)宋永安3)王 淙1)
1)郑州大学药物研究院 郑州 450001 2)青岛大学附属医院药剂科 山东青岛 266033 3)郑州大学人民医院呼吸内科 郑州 450003
Author(s):
ZHANG Xueyan1)LI Leilei1)SHI Xiaoli2)HUO Junfeng1) LIU Xiaojie1) QIN Tiantian1) LIU Weihua1) SHEN Yiwei1)SONG Yong'an3) WANG Cong1)
1)College of Pharmacy, Zhengzhou University, Zhengzhou 450001 2)Department of Pharmacy,the Affiliated Hospital of Qingdao University, Qingdao,Shandong 266033 3)Department of Respiration,the Affiliated People's Hospital of Zhengzhou University, Zhengzhou 450003
关键词:
喉癌 木犀草素 凋亡
Keywords:
laryngeal cancer luteolin apoptosis
分类号:
R739.91
DOI:
10.13705/j.issn.1671-6825.2018.12.027
摘要:
目的:研究木犀草素对喉癌Hep2细胞凋亡的影响及相关的作用机制。方法:磺酰罗丹明B(SRB)染色法检测0.5、1.0、2.0、4.0、8.0、16.0、32.0、64.0和128.0 μmol/L木犀草素作用24、48、72 h后Hep2细胞存活率; Hep2细胞分别用0、10、20和40 μmol/L木犀草素处理,流式细胞术检测各组Hep2细胞凋亡情况,Hoechst染色检测Hep2细胞中凋亡小体,Western blot实验检测凋亡相关蛋白Bax、Bcl-2、Bid的表达。结果:木犀草素呈时间浓度依赖性地抑制Hep2细胞存活(P<0.001)。木犀草素可以促进Hep2细胞的凋亡,诱导Hep2细胞产生凋亡小体,且能明显上调Bax、Bid蛋白的表达,降低Bcl-2蛋白的表达(P均<0.001)。结论:木犀草素通过上调Bax、Bid蛋白的表达和降低Bcl-2蛋白的表达诱导Hep2细胞的凋亡。
Abstract:
Aim:To study the effects of luteolin on the apoptosis of laryngeal cancer Hep2 cells and the mechanism.Methods:The survival of Hep2 cells treated by different concentrations(0.5,1.0,2.0,4.0,8.0,16.0,32.0,64.0,128.0 μmol/L )of luteolin after 24,48 and 72 hours was detected by SRB staining. The effects of different concentrations(0,10,20,40 μmol/L)of luteolin on apoptosis of Hep2 cells was detected by flow cytometry. Hoechst staining was used to detect the apoptotic body in Hep2 cells. The expressions of Bax, Bcl-2,and Bid proteins were detected by Western blot.Results:Luteolin significantly inhibited Hep2 cells survival in a time- and concentration-dependent manner(P<0.001). Luteolin induced apoptosis and apoptotic bodies were observed in Hep2 cells. Western blot showed that luteolin increased the expressions of Bax and Bid, while reduced the expression of Bcl-2(P<0.001).Conclusion:Luteolin induces apoptosis of Hep2 cells by upregulating Bax and Bid proteins and downregulating Bcl-2 protein.

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备注/Memo

备注/Memo:
【基金项目】河南省基础与前沿技术研究项目(152300410030); 河南省高等学校重点科研项目(18A350013, 14B350011) 【作者简介】王淙,通信作者,女,1972年10月生,博士,副教授,研究方向:肿瘤药理,E-mail:wangcong@zzu.edu.cn
更新日期/Last Update: 2019-11-20