[1]王树娟,范 熠,张 雨,等.过表达CSRP2对人B淋巴瘤细胞Ramos增殖、周期分布、凋亡及CREB表达的影响[J].郑州大学学报(医学版),2020,(06):771-774.[doi:10.13705/j.issn.1671-6825.2020.01.102]
 WANG Shujuan,FAN Yi,ZHANG Yu,et al.Effects of CSRP2 overexpression on human B lymphoma Ramos cells' proliferation,cell cycle,apoptosis and CREB expression[J].JOURNAL OF ZHENGZHOU UNIVERSITY(MEDICAL SCIENCES),2020,(06):771-774.[doi:10.13705/j.issn.1671-6825.2020.01.102]
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过表达CSRP2对人B淋巴瘤细胞Ramos增殖、周期分布、凋亡及CREB表达的影响()
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《郑州大学学报(医学版)》[ISSN:1671-6825/CN:41-1340/R]

卷:
期数:
2020年06期
页码:
771-774
栏目:
论著
出版日期:
2020-11-20

文章信息/Info

Title:
Effects of CSRP2 overexpression on human B lymphoma Ramos cells' proliferation,cell cycle,apoptosis and CREB expression
作者:
王树娟范 熠张 雨李梦亚王 冲刘 钰王伟琼郝倩倩刘延方
郑州大学第一附属医院血液科 郑州 450052
Author(s):
WANG ShujuanFAN YiZHANG YuLI MengyaWANG ChongLIU YuWANG WeiqiongHAO QianqianLIU Yanfang
Department of Hematology,the First Affiliated Hospital,Zhengzhou University,Zhengzhou 450052
关键词:
CSRP2 淋巴瘤 CREB信号通路 增殖 细胞周期 凋亡
Keywords:
CSRP2 lymphoma CREB signaling pathway proliferation cell cycle apoptosis
分类号:
R733
DOI:
10.13705/j.issn.1671-6825.2020.01.102
摘要:
目的:探讨过表达半胱氨酸和甘氨酸富集蛋白2(CSRP2)对人B淋巴瘤细胞Ramos增殖、周期分布、凋亡及CREB表达的影响。方法:通过慢病毒感染的方法构建CSRP2过表达的淋巴瘤Ramos细胞模型(过表达组),以感染对照慢病毒的Ramos细胞为对照。采用RT-PCR和Western blot检测感染效率。采用CCK-8法检测2组细胞增殖情况,采用流式细胞术和Annexin V/PI双染法分别检测细胞周期分布和凋亡,并用Western blot检测CREB的表达水平。结果:与对照组比较,过表达组CSRP2 mRNA和蛋白表达水平升高(P<0.05)。CSRP2过表达可促进细胞增殖和细胞周期进展(P<0.05),但对细胞凋亡无影响(P>0.05)。CSRP2过表达可显著上调CREB的磷酸化水平(P<0.05)。结论:CSRP2可能通过上调CREB的表达促进淋巴瘤细胞增殖。
Abstract:
Aim:To explore the effects of CSRP2 gene on the proliferation,cell cycle,apoptosis and CREB expression of human B lymphoma cell line Ramos.Methods:A CSRP2 overexpressing lymphoma cell model was constructed by lentivirus infection(overexpression group).The expression of CSRP2 in the 2 groups was verified by RT-PCR and Western blot; cell proliferation was detected by CCK-8 assay,and cell cycle and cell apoptosis were detected by flow cytometry and Annexin V/PI double staining.Western blot was used to detect the expression of CREB.Results: CSRP2 overexpressing lymphoma cell line was successfully constructed.CSRP2 overexpression could promote lymphoma cell line proliferation and cell cycle progression(P<0.05),but had no effect on apoptosis(P>0.05).CSRP2 overexpression could significantly increase the phosphorylation level of CREB(P<0.05).Conclusion: CSRP2 may promote lymphoma cell proliferation by upregulating the expression of CREB.

参考文献/References:

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备注/Memo

备注/Memo:
【基金项目】国家自然科学基金项目(81800137,U1804191)
【作者简介】刘延方,通信作者,男,1962年5月生,博士,教授,主任医师,研究方向:血液病的基础与临床,E-mail:fccliuyf1@zzu.edu.cn
更新日期/Last Update: 2020-11-20