[1]左淑飞),吕书龙),吴 洁),等.小核糖体管家基因RNA 1转染对IL-1β刺激的大鼠软骨细胞增殖及凋亡的影响[J].郑州大学学报(医学版),2020,(06):826-830.[doi:10.13705/j.issn.1671-6825.2019.12.143]
 ZUO Shufei),LYU Shulong),WU Jie),et al.Effects of transfecting SNHG1 on the proliferation and apoptosis of rat chondrocytes stimulated by IL-1β[J].JOURNAL OF ZHENGZHOU UNIVERSITY(MEDICAL SCIENCES),2020,(06):826-830.[doi:10.13705/j.issn.1671-6825.2019.12.143]
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小核糖体管家基因RNA 1转染对IL-1β刺激的大鼠软骨细胞增殖及凋亡的影响()
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《郑州大学学报(医学版)》[ISSN:1671-6825/CN:41-1340/R]

卷:
期数:
2020年06期
页码:
826-830
栏目:
应用研究
出版日期:
2020-11-20

文章信息/Info

Title:
Effects of transfecting SNHG1 on the proliferation and apoptosis of rat chondrocytes stimulated by IL-1β
作者:
左淑飞1)吕书龙2) 吴 洁1) 马 玲1) 付冬冬1) 范文强1)
1)新乡市中心医院风湿免疫科 河南新乡 453000 2)新乡市中医院风湿科 河南新乡 453000
Author(s):
ZUO Shufei1) LYU Shulong2) WU Jie1)MA Ling1)FU Dongdong1) FAN Wenqiang1)
1)Department of Rheumatology and Immunology,Xinxiang Central Hospital,Xinxiang,Henan 453000 2)Department of Rheumatology,Xinxiang Hospital of Traditional Chinese Medicine,Xinxiang,Henan 453000
关键词:
骨关节炎 小核糖体管家基因RNA 1 增殖 凋亡 PI3K/AKT信号通路
Keywords:
osteoarthritis small nucleolar RNA host gene 1 proliferation apoptosis PI3K/AKT signaling pathway
分类号:
R684.3
DOI:
10.13705/j.issn.1671-6825.2019.12.143
摘要:
目的:探讨转染小核糖体管家基因RNA 1(SNHG1)对IL-1β刺激的大鼠软骨细胞增殖、凋亡的影响及其机制。方法:以10 μg/L的IL-1β刺激大鼠软骨细胞6、12和24 h(以未刺激细胞为对照),采用实时荧光定量PCR法检测SNHG1的表达。将体外培养的软骨细胞分为正常对照组(正常培养)、IL-1β组(以10 μg/L IL-1β刺激24 h)、IL-1β+空载体组(转染pcDNA3.1空载体后以10 μg/L IL-1β刺激24 h)和IL-1β+SNHG1组(转染pcDNA3.1-SNHG1后以10 μg/L IL-1β刺激24 h),采用实时荧光定量PCR法检测各组细胞中SNHG1的表达,MTT法和Annexin V-FITC/PI双染法检测细胞增殖和凋亡情况,Western blot法检测细胞中PI3K/AKT信号通路相关蛋白PI3K、AKT、p-AKT、Bcl-2和Bax的表达。结果:与未刺激细胞比较,IL-1β刺激6、12和24 h,大鼠软骨细胞中SNHG1表达水平逐渐降低(P<0.05)。与正常对照组比较,IL-1β组细胞中SNHG1表达水平,细胞增殖率和PI3K、p-AKT、Bcl-2蛋白的表达水平降低,细胞凋亡率和Bax蛋白表达水平升高(P<0.05); 与IL-1β组或IL-1β+空载体组比较,IL-1β+SNHG1组细胞中SNHG1表达水平,细胞增殖率和PI3K、p-AKT、Bcl-2蛋白的表达水平均升高,而细胞凋亡率和Bax蛋白表达水平降低(P<0.05)。结论:SNHG1可促进IL-1β刺激的大鼠软骨细胞增殖并抑制其凋亡,其作用机制可能与激活PI3K/AKT信号通路有关。
Abstract:
Aim:To investigate the effects of long non-coding RNA(lncRNA)small nucleolar RNA host gene 1(SNHG1)on proliferation and apoptosis of IL-1β stimulated rat chondrocytes and its mechanism.Methods:Rat chondrocytes were stimulated with IL-1β at 10 μg/L for 6,12,and 24 hours(chondrocytes not stimulated by IL-1β were the control),and then the expression of SNHG1 was detected by real-time fluorescent quantitative PCR.Chondrocytes cultured in vitro were allocated into normal control group(normal culture),IL-1β group(stimulated with 10 μg/L IL-1β for 24 hours),IL-1β+empty vector group(transfected with pcDNA3.1 empty vector and stimulated with 10 μg/L IL-1β for 24 hours),and IL-1β+SNHG1 group(transfected with pcDNA3.1-SNHG1 and stimulated with 10 μg/L IL-1β for 24 hours).The expression of SNHG1 in cells of each group was detected by real-time fluorescent quantitative PCR.Cell proliferation and apoptosis were detected by MTT assay and Annexin V-FITC/PI double staining.The expressions of PI3K/AKT signaling pathway related proteins such as PI3K,AKT,p-AKT,Bcl-2 and Bax were tested by Western blot.Results:After IL-1β stimulation for 6,12 and 24 hours,the expression level of SNHG1 in rat chondrocytes decreased gradually compared with the non-stimulated group(P<0.05).Compared with normal control group,the expression level of SNHG1,cell proliferation rate and the expression levels of PI3K,p-AKT,Bcl-2 protein in IL-1β group were significantly decreased,while apoptosis rate and the expression level of Bax protein were significantly increased(P<0.05); compared with IL-1β group or IL-1β+empty vector group,the expression level of SNHG1,cell proliferation rate and the expression levels of PI3K,p-AKT,Bcl-2 protein in IL-1β+SNHG1 group were significantly higher,while the apoptosis rate and Bax protein expression level were significantly lower(P<0.05).Conclusions:SNHG1 can promote IL-1β stimulated rat chondrocytes'proliferation and inhibit apoptosis,and the mechanism may be related to the activation of PI3K/AKT signaling pathway.

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备注/Memo

备注/Memo:
【基金项目】河南省卫生厅科技攻关计划项目(142300410098)
【作者简介】范文强,通信作者,男,1981年11月生,硕士,副主任医师,研究方向:自身免疫性疾病的诊断与治疗,E-mail:fwq.1125@163.com
更新日期/Last Update: 2020-11-20