[1]左淑飞),吕书龙),吴 洁),等.小核糖体管家基因RNA 1转染对IL-1β刺激的大鼠软骨细胞增殖及凋亡的影响[J].郑州大学学报(医学版),2020,(06):826-830.[doi:10.13705/j.issn.1671-6825.2019.12.143]
　ZUO Shufei),LYU Shulong),WU Jie),et al.Effects of transfecting SNHG1 on the proliferation and apoptosis of rat chondrocytes stimulated by IL-1β[J].JOURNAL OF ZHENGZHOU UNIVERSITY(MEDICAL SCIENCES),2020,(06):826-830.[doi:10.13705/j.issn.1671-6825.2019.12.143]
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小核糖体管家基因RNA 1转染对IL-1β刺激的大鼠软骨细胞增殖及凋亡的影响()
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《郑州大学学报(医学版)》[ISSN:1671-6825/CN:41-1340/R]

卷:
期数:
2020年06期
页码:
826-830
栏目:
应用研究
出版日期:
2020-11-20

文章信息/Info

Title:
Effects of transfecting SNHG1 on the proliferation and apoptosis of rat chondrocytes stimulated by IL-1β
作者:
左淑飞1); 吕书龙2);  吴 洁1);  马 玲1);  付冬冬1);  范文强1)
1)新乡市中心医院风湿免疫科 河南新乡 453000 2)新乡市中医院风湿科 河南新乡 453000
Author(s):
ZUO Shufei1);  LYU Shulong2);  WU Jie1); MA Ling1); FU Dongdong1);  FAN Wenqiang1)
1)Department of Rheumatology and Immunology,Xinxiang Central Hospital,Xinxiang,Henan 453000 2)Department of Rheumatology,Xinxiang Hospital of Traditional Chinese Medicine,Xinxiang,Henan 453000
关键词:
骨关节炎;  小核糖体管家基因RNA 1;  增殖;  凋亡;  PI3K/AKT信号通路
Keywords:
osteoarthritis;  small nucleolar RNA host gene 1;  proliferation;  apoptosis;  PI3K/AKT signaling pathway
分类号:
R684.3
DOI:
10.13705/j.issn.1671-6825.2019.12.143
摘要:
目的:探讨转染小核糖体管家基因RNA 1(SNHG1)对IL-1β刺激的大鼠软骨细胞增殖、凋亡的影响及其机制。方法:以10 μg/L的IL-1β刺激大鼠软骨细胞6、12和24 h(以未刺激细胞为对照),采用实时荧光定量PCR法检测SNHG1的表达。将体外培养的软骨细胞分为正常对照组(正常培养)、IL-1β组(以10 μg/L IL-1β刺激24 h)、IL-1β+空载体组(转染pcDNA3.1空载体后以10 μg/L IL-1β刺激24 h)和IL-1β+SNHG1组(转染pcDNA3.1-SNHG1后以10 μg/L IL-1β刺激24 h),采用实时荧光定量PCR法检测各组细胞中SNHG1的表达,MTT法和Annexin V-FITC/PI双染法检测细胞增殖和凋亡情况,Western blot法检测细胞中PI3K/AKT信号通路相关蛋白PI3K、AKT、p-AKT、Bcl-2和Bax的表达。结果:与未刺激细胞比较,IL-1β刺激6、12和24 h,大鼠软骨细胞中SNHG1表达水平逐渐降低(P<0.05)。与正常对照组比较,IL-1β组细胞中SNHG1表达水平,细胞增殖率和PI3K、p-AKT、Bcl-2蛋白的表达水平降低,细胞凋亡率和Bax蛋白表达水平升高(P<0.05); 与IL-1β组或IL-1β+空载体组比较,IL-1β+SNHG1组细胞中SNHG1表达水平,细胞增殖率和PI3K、p-AKT、Bcl-2蛋白的表达水平均升高,而细胞凋亡率和Bax蛋白表达水平降低(P<0.05)。结论:SNHG1可促进IL-1β刺激的大鼠软骨细胞增殖并抑制其凋亡,其作用机制可能与激活PI3K/AKT信号通路有关。
Abstract:
Aim:To investigate the effects of long non-coding RNA(lncRNA)small nucleolar RNA host gene 1(SNHG1)on proliferation and apoptosis of IL-1β stimulated rat chondrocytes and its mechanism.Methods:Rat chondrocytes were stimulated with IL-1β at 10 μg/L for 6,12,and 24 hours(chondrocytes not stimulated by IL-1β were the control),and then the expression of SNHG1 was detected by real-time fluorescent quantitative PCR.Chondrocytes cultured in vitro were allocated into normal control group(normal culture),IL-1β group(stimulated with 10 μg/L IL-1β for 24 hours),IL-1β+empty vector group(transfected with pcDNA3.1 empty vector and stimulated with 10 μg/L IL-1β for 24 hours),and IL-1β+SNHG1 group(transfected with pcDNA3.1-SNHG1 and stimulated with 10 μg/L IL-1β for 24 hours).The expression of SNHG1 in cells of each group was detected by real-time fluorescent quantitative PCR.Cell proliferation and apoptosis were detected by MTT assay and Annexin V-FITC/PI double staining.The expressions of PI3K/AKT signaling pathway related proteins such as PI3K,AKT,p-AKT,Bcl-2 and Bax were tested by Western blot.Results:After IL-1β stimulation for 6,12 and 24 hours,the expression level of SNHG1 in rat chondrocytes decreased gradually compared with the non-stimulated group(P<0.05).Compared with normal control group,the expression level of SNHG1,cell proliferation rate and the expression levels of PI3K,p-AKT,Bcl-2 protein in IL-1β group were significantly decreased,while apoptosis rate and the expression level of Bax protein were significantly increased(P<0.05); compared with IL-1β group or IL-1β+empty vector group,the expression level of SNHG1,cell proliferation rate and the expression levels of PI3K,p-AKT,Bcl-2 protein in IL-1β+SNHG1 group were significantly higher,while the apoptosis rate and Bax protein expression level were significantly lower(P<0.05).Conclusions:SNHG1 can promote IL-1β stimulated rat chondrocytes'proliferation and inhibit apoptosis,and the mechanism may be related to the activation of PI3K/AKT signaling pathway.

参考文献/References:

[1] HAWKER GA.Osteoarthritis is a serious disease[J].Clin Exp Rheumatol,2019,Suppl 120(5):3
[2] 杨长生,蒋家望,段志宏,等.综合康复疗法对老年膝关节骨关节炎的疗效观察[J].解放军医学杂志,2004,29(5):391
[3] 毛建杰,徐兵,王庆.全膝关节置换术对老年膝骨关节炎患者的疗效及生活质量的影响[J].实用临床医药杂志,2018,22(1):84
[4] 王晶,胡敏霞.肌骨超声技术观察针刀治疗膝骨关节炎患者的临床效果与影像学变化[J].医学影像学杂志,2018,28(9):1544
[5] 冯俊奇,孙超,孙军强,等.药透治疗对兔膝骨关节炎软骨细胞凋亡和相关基因表达的影响[J].解放军医学杂志,2009,34(2):186
[6] LEI J,FU Y,ZHUANG Y,et al.LncRNA SNHG1 alleviates IL-1β-induced osteoarthritis by inhibiting miR-16-5p-mediated p38 MAPK and NF-κB signaling pathways[J].Biosci Rep,2019,39(9):BSR20191523
[7] HUANG T,WANG J,ZHOU Y,et al.LncRNA CASC2 is up-regulated in osteoarthritis and participates in the regulation of IL-17 expression and chondrocyte proliferation and apoptosis[J].Biosci Rep,2019,39(5):BSR20182454
[8] LI L,LV G,WANG B,et al.The role of lncRNA XIST/miR-211 axis in modulating the proliferation and apoptosis of osteoarthritis chondrocytes through CXCR4 and MAPK signaling[J].Biochem Biophys Res Commun,2018,503(4):2555
[9] LI Y,LI S,LUO Y,et al.LncRNA PVT1 regulates chondrocyte apoptosis in osteoarthritis by acting as a sponge for miR-488-3p[J].DNA Cell Biol,2017,36(7):571
[10]ZHANG G,WU Y,XU D,et al.Long noncoding RNA UFC1 promotes proliferation of chondrocyte in osteoarthritis by acting as a sponge for miR-34a[J].DNA Cell Biol,2016,35(11):691
[11]LU Q,SHAN S,LI Y,et al.Long noncoding RNA SNHG1 promotes non-small cell lung cancer progression by up-regulating MTDH via sponging miR-145-5p[J].FASEB J,2018,32(7): 3957
[12]ZHANG N,MENG X,MEI L,et al.The long non-coding RNA SNHG1 attenuates cell apoptosis by regulating miR-195 and BCL2-like protein 2 in human cardiomyocytes[J].Cell Physiol Biochem,2018,50(3): 1029
[13]李坤,张育民,王亚康,等.上调microRNA-543表达对大鼠骨关节炎软骨细胞的保护作用[J].中国临床医学,2018,25(2):239
[14]YANG Y,WANG Y,ZHAO M,et al.Tormentic acid inhibits IL-1β-induced chondrocyte apoptosis by activating the PI3K/Akt signaling pathway[J].Mol Med Rep,2018,17(3): 4753
[15]董良杰,王勤俭,王单一,等.白芍总苷对人骨关节炎软骨细胞增殖及p38MAPK蛋白表达的影响[J].郑州大学学报(医学版),2019,54(3):431
[16]郑欣鹏.尼古丁通过PI3K/Akt信号通路保护大鼠软骨细胞抵抗白介素-1诱导的细胞凋亡[D].厦门:厦门大学,2012.
[17]CHEN J,ZHANG W,WU YQ,et al.LncRNA SNHG1 inhibits neuronal apoptosis in cerebral infarction rats through PI3K/Akt signaling pathway[J].Eur Rev Med Pharmacol Sci,2019,23(12): 5366
[18]ZHANG Y,ZHANG R,LUO G,et al.Long noncoding RNA SNHG1 promotes cell proliferation through PI3K/AKT signaling pathway in pancreatic ductal adenocarcinoma[J].J Cancer,2018,9(15): 2713

备注/Memo

备注/Memo:
【基金项目】河南省卫生厅科技攻关计划项目(142300410098)
【作者简介】范文强,通信作者,男,1981年11月生,硕士,副主任医师,研究方向:自身免疫性疾病的诊断与治疗,E-mail:fwq.1125@163.com
更新日期/Last Update: 2020-11-20