[1]吴 洁),马 玲),付冬冬),等.调控类风湿关节炎PBMC中miR-142-3p的表达对ADAM17表达及成纤维样滑膜细胞活性的影响[J].郑州大学学报(医学版),2020,(06):844-848.[doi:10.13705/j.issn.1671-6825.2019.09.162]
　WU Jie),MA Ling),FU Dongdong),et al.Effects of regulating miR-142-3p in PBMC from patients with rheumatoid arthritis on ADAM17 expression and proliferation of fibroblast-like synovial cells[J].JOURNAL OF ZHENGZHOU UNIVERSITY(MEDICAL SCIENCES),2020,(06):844-848.[doi:10.13705/j.issn.1671-6825.2019.09.162]
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调控类风湿关节炎PBMC中miR-142-3p的表达对ADAM17表达及成纤维样滑膜细胞活性的影响()
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《郑州大学学报(医学版)》[ISSN:1671-6825/CN:41-1340/R]

卷:
期数:
2020年06期
页码:
844-848
栏目:
应用研究
出版日期:
2020-11-20

文章信息/Info

Title:
Effects of regulating miR-142-3p in PBMC from patients with rheumatoid arthritis on ADAM17 expression and proliferation of fibroblast-like synovial cells
作者:
吴 洁1); 马 玲1); 付冬冬1); 杨学华2); 吕书龙3); 范文强1); 王培山4)
1)新乡市中心医院风湿免疫科 河南新乡 453000 2)新乡市第二人民医院肾病风湿科 河南新乡 453000 3)新乡市中医院风湿科 河南新乡 453000 4)新乡市中心医院麻醉手术部 河南新乡 453000
Author(s):
WU Jie1); MA Ling1); FU Dongdong1); YANG Xuehua2); LYU Shulong3); FAN Wenqiang1); WANG Peishan4)
1)Department of Rheumatology and Immunology,Xinxiang Central Hospital,Xinxiang,Henan 453000 2)Department of Nephropathy and Rheumatism,Xinxiang Second People's Hospital,Xinxiang,Henan 453000 3)Department of Rheumatology,Xinxiang Hospital of Traditional Chinese Medicine,Xinxiang,Henan 453000 4)Department of Anesthesia Operation,Xinxiang Central Hospital,Xinxiang,Henan 453000
关键词:
类风湿关节炎;  成纤维样滑膜细胞;  miR-142-3p;  ADAM17;  细胞增殖;  细胞凋亡
Keywords:
rheumatoid arthritis;  fibroblast-like synovial cell;  miR-142-3p;  ADAM17;  proliferation;  apoptosis
分类号:
R593.22
DOI:
10.13705/j.issn.1671-6825.2019.09.162
摘要:
目的:探讨类风湿关节炎(RA)外周血单个核细胞(PBMC)中微小RNA-142-3p(miR-142-3p)的表达对RA成纤维样滑膜细胞(FLS)增殖和凋亡的影响。方法:采用生物信息学预测和双荧光素酶报告实验分析miR-142-3p和解聚素金属蛋白酶17(ADAM17)的靶向关系。分别采用实时荧光定量PCR法和Western blot法检测人正常PBMC和RA-PBMC中miR-142-3p和ADAM17 蛋白的表达。利用miR-142-3p模拟物或抑制物转染调控RA-PBMC中miR-142-3p的表达,观察低表达miR-142-3p,及过表达ADAM17和miR-142-3p的RA-PBMC对RA-FLS增殖和凋亡的影响。RA-FLS增殖的检测采用MTT法,RA-FLS凋亡的检测采用Annexin V-FITC和PI双染法。结果:ADAM17和miR-142-3p存在靶向关系。与正常PBMC相比, RA-PBMC中miR-142-3p表达上调,ADAM17蛋白表达下调(P<0.05)。低表达miR-142-3p的RA-PBMC和过表达ADAM17的RA-PBMC均可增强RA-FLS增殖活性,减少其凋亡(P<0.05)。过表达miR-142-3p的RA-PBMC可降低RA-FLS的增殖活性,促进其凋亡(P<0.05)。过表达ADAM17 和过表达miR-142-3p的作用可以相互拮抗(P<0.05)。结论:调控RA-PBMC中miR-142-3p的表达可以通过调控ADAM17而影响RA-FLS的增殖和凋亡。
Abstract:
Aim:To investigate the effects of miR-142-3p expression in peripheral blood mononuclear cells(PBMC)from the patients with rheumatoid arthritis(RA)on the proliferation and apoptosis of fibroblast-like synovial cells(FLS)of RA patients.Methods:The targeting relationship between miR-142-3p and metalloproteinase 17(ADAM17)was analyzed by biological information prediction and double luciferase report test.The expressions of miR-142-3p and ADAM17 in normal PBMC and RA-PBMC were detected by real-time quantitative PCR and Western blot,respectively.miR-142-3p expression in RA-PBMC was regulated by miR-142-3p analogue or inhibitor transfection.The effects of RA-PBMC with low miR-142-3p expression,or overexpression of ADAM17 and miR-142-3p on proliferation and apoptosis of RA-FLS were observed.The proliferation of RA-FLS was detected by MTT assay,and the apoptosis of RA-FLS was detected by Annexin V-FITC and PI double staining.Results:There was a targeted relationship between ADAM17 and MIR-142-3P.Compared with normal PBMC,the expression of miR-142-3p in RA-PBMC was higher,and that of ADAM17 was lower(P<0.05).The proliferation of RA-FLS induced by RA-PBMC with low expression of miR-142-3p or overexpression of ADAM17 decreased and the apoptosis rate increased(P<0.05).The proliferation of RA-FLS induced by RA-PBMC with overexpression of miR-142-3p increased,the apoptosis rate decreased(P<0.05).Overexpression of ADAM17 and overexpression of miR-142-3p could antagonize each other(P<0.05).Conclusion:Modulating the expression of miR-142-3p in RA-PBMC could affect the proliferation and apoptosis of RA-FLS through modulating ADAM17.

参考文献/References:

[1] ANGELOTTI F,PARMA A,CAFARO G,et al.One year in review 2017:pathogenesis of rheumatoid arthritis[J].Clin Exp Rheumatol,2017,35(3):368
[2] 尹芳蕊,庞春艳,耿立霞,等.过表达miR-124a对J774.1细胞中TNF-α和IL-6表达水平及细胞周期的影响[J].吉林大学学报(医学版),2018,44(5):983
[3] ASIF AMIN M,FOX DA,RUTH JH.Synovial cellular and molecular markers in rheumatoid arthritis[J].Semir Immunopathol,2017,39(4):385
[4] 郑洋洋,代东雪,潘志,等.红景天苷对TNF-α诱导的人类风湿关节炎成纤维样滑膜细胞增殖的抑制作用及其意义[J].吉林大学学报(医学版),2017,43(3):485
[5] WANG X,GUO Y,WANG C,et al.MicroRNA-142-3p inhibits chondrocyte apoptosis and inflammation in osteoarthritis by targeting HMGB1[J].Inflammation,2016,39(5):1718
[6] HONG BK,YOU SY,YOO SA,et al.MicroRNA-143 and -145 modulate the phenotype of synovial fibroblasts in rheumatoid arthritis[J].Exp Mol Med,2017,49(8):e363
[7] LAI NS,YU HC,TUNG CH,et al.The role of aberrant expression of T cell miRNAs affected by TNF-α in the immunopathogenesis of rheumatoid arthritis[J].Arthritis Res Ther,2017,19(1): 261
[8] 赵涌,靳慧芳,韦利强,等.类风湿性关节炎患者外周血单个核细胞中miR-142-3p的表达及临床意义[J].中国卫生检验杂志,2015,25(20):3514
[9] GEBREMARIAM HG,QAZI KR,SOMIAH T,et al.Lactobacillus gasseri suppresses the production of the proinflammatory cytokines in Helicobacter pylori-infected macrophages by inhibiting the expression of ADAM17[J].Front Immunol,2019,10:2326
[10]SAAD MI,ROSE-JOHN S,JENKINS BJ.ADAM17:an emerging therapeutic target for lung cancer[J].Cancers(Basel),2019,11(9):1218
[11]CALABRESI E,PETRELLI F,BONIFACIO AF,et al.One year in review 2018:pathogenesis of rheumatoid arthritis[J].Clin Exp Rheumatol,2018,36(2):175
[12]HANAOKA BY,ITHURBURN MP,RIGSBEE CA,et al.Chronic inflammation in rheumatoid arthritis and mediators of skeletal muscle pathology and physical impairment: a review[J].Arthritis Care Res(Hoboken),2019,71(2):173
[13]胡君,汤自征,张育,等.正常和类风湿关节炎关节成纤维样滑膜细胞迁移、侵袭能力的差异[J].中国组织工程研究,2017,21(24):3870
[14]PAN BL,TONG ZW,WU L,et al.Effects of microRNA-206 on osteosarcoma cell proliferation,apoptosis,migration and invasion by targeting ANXA2 through the AKT signaling pathway[J].Cell Physiol Biochem,2018,45(4):1410
[15]TAVASOLIAN F,ABDOLLAHI E,REZAEI R,et al.Altered expression of microRNAs in rheumatoid arthritis[J].J Cell Biochem,2018,119(1):478
[16]YANG J,ZHUANG YX,LIU JH.Upregulation of microRNA-590 in rheumatoid arthritis promotes apoptosis of bone cells through transforming growth factor-β1/phosphoinositide 3-kinase/Akt signaling[J].Int J Mol Med,2019,43(5):2212
[17]ABDUL-MAKSOUD RS,SEDIQ AM,KATTAIA A,et al.Serum miR-210 and miR-155 expression levels as novel biomarkers for rheumatoid arthritis diagnosis[J].Br J Biomed Sci,2017,74(4):209
[18]LI SP,JIN ZM,LU XB.MicroRNA-192 suppresses cell proliferation and induces apoptosis in human rheumatoid arthritis fibroblast-like synoviocytes by downregulating caveolin 1[J].Mol Cell Biochem,2017,432(1/2):123
[19]ZHANG B,WANG LS,ZHOU YH.Elevated microRNA-125b promotes inflammation in rheumatoid arthritis by activation of NF-κB pathway[J].Biomed Pharmacother,2017,93:1151
[20]张琪,杨光华,黄志强,等.沉默ADAM17对HT29人结肠癌细胞体外侵袭能力的影响及机制探究[J].中国现代医学杂志,2019,29(7): 24
[21]MATTHEWS AL,NOY PJ,REYAT JS,et al.Regulation of adisintegrin and metalloproteinase(ADAM)family sheddases Adam10 and Adam17:the emerging role of tetraspanins and rhomboids[J].Platelets,2017,28(4):333
[22]ZUNKE F,ROSE-JOHN S.The shedding protease ADAM17:physiology and pathophysiology[J].Biochim Biophys Acta Mol Cell Res,2017,1864(11 Pt B):2059

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备注/Memo

备注/Memo:
【作者简介】王培山,通信作者,男,1966年1月生,硕士,主任医师,研究方向:类风湿关节炎,E-mail:ccff-218000@163.com
更新日期/Last Update: 2020-11-20