[1]李碧军),郭瑞霞),王春芳),等.p16/Ki67双染、HPV E6/E7 mRNA检测对于TCT检查为低级别鳞状上皮内病变及以下且HPV检测为阴性或其他高危型HPV阳性患者的分流作用[J].郑州大学学报(医学版),2021,(01):11-15.
 LI Bijun),GUO Ruixia),WANG Chunfang),et al.Role of p16/Ki67 dual staining and HPV E6/E7 mRNA detection for triage in patients with LSIL or lower lesions diagnosed by TCT and HPV negative or other high-risk HPV positive[J].JOURNAL OF ZHENGZHOU UNIVERSITY(MEDICAL SCIENCES),2021,(01):11-15.
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p16/Ki67双染、HPV E6/E7 mRNA检测对于TCT检查为低级别鳞状上皮内病变及以下且HPV检测为阴性或其他高危型HPV阳性患者的分流作用()
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《郑州大学学报(医学版)》[ISSN:1671-6825/CN:41-1340/R]

卷:
期数:
2021年01期
页码:
11-15
栏目:
论著
出版日期:
2021-01-20

文章信息/Info

Title:
Role of p16/Ki67 dual staining and HPV E6/E7 mRNA detection for triage in patients with LSIL or lower lesions diagnosed by TCT and HPV negative or other high-risk HPV positive
作者:
李碧军1)郭瑞霞1)王春芳1)陈 曦1)褚丹霞1)王凯丽2)杜建敏1)白 晶1)
1)郑州大学第一附属医院妇科 郑州 450052 2)郑州大学第三附属医院妇科 郑州 450052
Author(s):
LI Bijun1) GUO Ruixia1) WANG Chunfang1) CHEN Xi1)CHU Danxia1) WANG Kaili2) DU Jianmin1) BAI Jing1)
1)Department of Gynecology,the First Affiliated Hospital,Zhengzhou University,Zhengzhou 450052 2)Department of Gynecology,the Third Affiliated Hospital,Zhengzhou University,Zhengzhou 450052
关键词:
TCT p16/Ki67双染色法 高危HPV E6/E7 mRNA 宫颈癌 联合检测
Keywords:
TCT p16/Ki67 dual staining high-risk HPV E6/E7 mRNA cervical cancer combined detection
分类号:
R737.33
摘要:
目的:探索p16/Ki67双染色法(简称双染)、HPV E6/E7 mRNA(简称E6/E7)检测对于宫颈细胞学检查(TCT)为低级别鳞状上皮内病变(LSIL)及以下且HPV检测为阴性或其他高危型HPV阳性患者的分流作用。方法:收集郑州大学第一附属医院妇科门诊就诊的TCT检查为LSIL及以下且HPV检测为阴性或其他高危型HPV阳性(不包括HPV16、18阳性)的414例患者的宫颈脱落细胞,分别进行双染检测、HPV E6/E7 mRNA检测,并行阴道镜下活检,以组织病理学诊断为“金标准”,采用灵敏度、特异度比较TCT+HPV+双染、TCT+HPV+E6/E7和TCT+HPV检测用于筛查高级别鳞状上皮内病变及以上(HSIL+)患者的效能。结果:根据病理结果,414例中150例为HSIL+。TCT+HPV+双染检出宫颈HSIL+病变的灵敏度、特异度分别为78.7%、50.4%,TCT+HPV+E6/E7分别为74.0%、26.1%,而TCT+HPV为90.0%、8.0%。TCT+HPV+双染和TCT+HPV+E6/E7检出HSIL+病变的灵敏度相近,均略低于TCT+HPV(P<0.05),而TCT+HPV+双染的特异度远高于其他2种方法(P<0.05)。结论:对于TCT检查为LSIL及以下且HPV阴性或其他高危型HPV阳性患者,采用p16/Ki67双染联合检测具有较高的灵敏度,且能显著提高特异度,其筛查效能优于TCT和HPV联合筛查。
Abstract:
Aim:To investigate the application value of p16/Ki67 dual staining(dual staining)and HPV E6/E7 mRNA(E6/E7)detection for triage in patients with LSIL or lower lesions diagnosed by TCT and HPV negative or other high-risk HPV positive.Methods:A total of 414 patients with LSIL or lower lesions diagnosed by TCT and HPV negative or other high-risk HPV positive admitted by the First Affiliated Hospital of Zhengzhou University were collected. Cervical exfoliated cells were detected by dual staining and E6/E7 detection, and colposcopy biopsy was also performed. Taking histopathology as the gold standard,the sensitivity and specificity of TCT+HPV+dual staining,TCT+HPV+E6/E7,and TCT+HPV for diagnosing HSIL or higher lesion(HSIL+)were compared.Results:According to the results of biopsy, there were 150 cases of HSIL+ among 414 cases.The sensitivity and specificity of TCT+HPV+dual staining for detecting HSIL+ were 78.7% and 50.4%, those for TCT+HPV+E6/E7 were 74.0% and 26.1%,and those for TCT+HPV were 90.0% and 8.0%.The sensitivity of TCT+HPV+dual staining and TCT+HPV+E6/E7 were similar,lower than that of TCT+HPV(P<0.05), while the specificity of TCT+HPV+dual staining was higher than those of TCT+HPV+E6/E7 and TCT+HPV(P<0.05).Conclusion:When the screening results of cervical cancer are LSIL or lower lesions diagnosed by TCT and HPV negative or other high-risk HPV positive, p16/Ki67 dual staining combined detection can maintain high sensitivity and improve the specificity; its screening efficiency is better than TCT+HPV combined screening.

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备注/Memo

备注/Memo:
【基金项目】国家自然科学基金面上项目(31670844); 中原千人计划-中原名医项目(ZYQR201810107); 河南省科技创新杰出人才项目(2018JR0004); 河南省医学科技攻关计划项目(省部共建)(SBGJ2018005)
【作者简介】郭瑞霞,通信作者,女,1972年3月生,博士,教授,研究方向:妇科肿瘤,E-mail: grxcdxzzu@163.com
更新日期/Last Update: 2021-02-15