[1]郭慧丽)△,张明昌),郑乃刚),等.纳米脂质体槲皮素联合8BrcAMP对Rb44细胞凋亡的影响*[J].郑州大学学报(医学版),2011,(03):393.
 GAO Huili,ZHANG Mingchang,ZHENG Naigang),et al.Effects of nanoliposomal quercetin combined with 8BrcAMP on apoptosis of Rb44 cells[J].JOURNAL OF ZHENGZHOU UNIVERSITY(MEDICAL SCIENCES),2011,(03):393.
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纳米脂质体槲皮素联合8BrcAMP对Rb44细胞凋亡的影响*
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《郑州大学学报(医学版)》[ISSN:1671-6825/CN:41-1340/R]

卷:
期数:
2011年03期
页码:
393
栏目:
论著
出版日期:
2011-05-15

文章信息/Info

Title:
Effects of nanoliposomal quercetin combined with 8BrcAMP on apoptosis of Rb44 cells
作者:
郭慧丽1)△张明昌1)郑乃刚2)吴景兰2)王爱凤3)
1)华中科技大学同济医学院附属协和医院眼科 武汉430032
2)郑州大学分子细胞生物学研究中心 郑州4500013)河南省人民医院眼科 郑州 450003
Author(s):
GAO Huili1)ZHANG Mingchang1)ZHENG Naigang2)WU Jinglan2)WANG Aifeng3)
1)Department of Ophthalmology,the Affiliated Xiehe Hospital,Tongji Medical College, Huazhong University,Technology and Science,Wuhan 430032
2)Molecular Cell Biology Research Center, Zhengzhou University, Zhengzhou 450001
3)Department of Ophthalmology, Henan People’s Hospital, Zhengzhou 450003
关键词:
凋亡 纳米脂质体槲皮素 8BrcAMPRb44 细胞
分类号:
R739.703
文献标志码:
A
摘要:
目的:探讨纳米脂质体槲皮素(nLQ)和8BrcAMP(Br)对人视网膜母细胞瘤Rb44 细胞凋亡和p53、p21waf1 mRNA表达及Bcl2、 Bax和Caspase3蛋白表达的影响。方法:Rb44 细胞分为4组:nLQ组给予终浓度为40 μmol/L nLQ;Br组给予终浓度为2×10-5 mol/L Br,联合用药组给予同上浓度的nLQ和Br,对照组不给药。采用TUNEL法检测各组细胞的凋亡率。采用原位杂交技术检测p53、p21waf1 mRNA的表达,免疫细胞化学方法检测Bcl2、Bax及Caspase3蛋白的表达。结果:与对照组相比,单用nLQ或 Br均可增高凋亡率,上调p53和p21waf1 mRNA的表达及Bax和Caspase3蛋白的表达,下调Bcl2蛋白的表达(P<0.001); nLQ及Br联用呈现协同效应(P<0.001)。结论:nLQ 和8BrcAMP可能通过上调p53和p21 waf1基因的表达,诱导线粒体途径和Caspase3的最终共同凋亡途径而诱导Rb44细胞趋向凋亡,且两药联用呈现协同效应。

参考文献/References:

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备注/Memo

备注/Memo:
 *河南省科技厅基础与前沿技术研究计划基金资助项目102300410025
△女,1965年5月生,硕士,主任医师,研究方向:眼底病的基础与临床,E-mail:gse_2005@126.com
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